LOCUS       sequence-296740-        4326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human b2AR in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Inagaki S, Iwata R, Iwamoto M, Imai T
  TITLE     Widespread Inhibition, Antagonism, and Synergy in Mouse Olfactory 
            Sensory Neurons In Vivo.
  JOURNAL   Cell Rep. 2020 Jun 30;31(13):107814. doi: 
            10.1016/j.celrep.2020.107814.
  PUBMED    32610120
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-06-30"; volume: "31(13):107814. doi"; pages: " 
            10.1016/j.celrep.2020.107814"
COMMENT     Sequence Label: pME18S-F-R-hb2AR
FEATURES             Location/Qualifiers
     source          1..4326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(18..347,348..363,364..632)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             364..632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             803..826
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             848..886
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     polyA_signal    2231..2365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2268..2287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2322..2341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2404..2421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2575..3163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2655..2674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3334..4125,4126..4194))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3957..3976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4195..4299)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat
      361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
      421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
      481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
      541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
      601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact
      661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag
      721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      781 tctaaaagct gcggaattca tggactacaa ggacgacgac gacaaggaat tcatgaacgg
      841 gaccgagggc ccaaacttct acgtgccttt ctccaacaag acgggcgtgg tgctcgagat
      901 ggggcaaccc gggaacggca gcgccttctt gctggcaccc aatggaagcc atgcgccgga
      961 ccacgacgtc acgcaggaaa gggacgaggt gtgggtggtg ggcatgggca tcgtcatgtc
     1021 tctcatcgtc ctggccatcg tgtttggcaa tgtgctggtc atcacagcca ttgccaagtt
     1081 cgagcgtctg cagacggtca ccaactactt catcacttca ctggcctgtg ctgatctggt
     1141 catgggcctg gcagtggtgc cctttggggc cgcccatatt cttatgaaaa tgtggacttt
     1201 tggcaacttc tggtgcgagt tttggacttc cattgatgtg ctgtgcgtca cggccagcat
     1261 tgagaccctg tgcgtgatcg cagtggatcg ctactttgcc attacttcac ctttcaagta
     1321 ccagagcctg ctgaccaaga ataaggcccg ggtgatcatt ctgatggtgt ggattgtgtc
     1381 aggccttacc tccttcttgc ccattcagat gcactggtac cgggccaccc accaggaagc
     1441 catcaactgc tatgccaatg agacctgctg tgacttcttc acgaaccaag cctatgccat
     1501 tgcctcttcc atcgtgtcct tctacgttcc cctggtgatc atggtcttcg tctactccag
     1561 ggtctttcag gaggccaaaa ggcagctcca gaagattgac aaatctgagg gccgcttcca
     1621 tgtccagaac cttagccagg tggagcagga tgggcggacg gggcatggac tccgcagatc
     1681 ttccaagttc tgcttgaagg agcacaaagc cctcaagacg ttaggcatca tcatgggcac
     1741 tttcaccctc tgctggctgc ccttcttcat cgttaacatt gtgcatgtga tccaggataa
     1801 cctcatccgt aaggaagttt acatcctcct aaattggata ggctatgtca attctggttt
     1861 caatcccctt atctactgcc ggagcccaga tttcaggatt gccttccagg agcttctgtg
     1921 cctgcgcagg tcttctttga aggcctatgg gaatggctac tccagcaacg gcaacacagg
     1981 ggagcagagt ggatatcacg tggaacagga gaaagaaaat aaactgctgt gtgaagacct
     2041 cccaggcacg gaagactttg tgggccatca aggtactgtg cctagcgata acattgattc
     2101 acaagggagg aattgtagta caaatgactc actgctgtaa ccgcgggcgg ccgctagact
     2161 agtctagaga aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     2221 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     2281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     2341 caatgtatct tatcatgtct ggatccccgg gtaccgagct cgaattaatt cctcttccgc
     2401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2761 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3001 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3541 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3601 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3661 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3721 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3781 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3841 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3901 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3961 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4021 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4081 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4141 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4201 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4261 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4321 acctgc
//
