LOCUS       sequence-296753-       10492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of N-terminal truncated version of native 
            3-hydroxy-3-methyl glutaryl CoA reductase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Jaramillo-Madrid AC, Abbriano R, Ashworth J, Fabris M, Pernice M, 
            Ralph PJ
  TITLE     Overexpression of Key Sterol Pathway Enzymes in Two Model Marine 
            Diatoms Alters Sterol Profiles in Phaeodactylum tricornutum.
  JOURNAL   Pharmaceuticals (Basel). 2020 Dec 21;13(12). pii: ph13120481. doi: 
            10.3390/ph13120481.
  PUBMED    33371196
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pharmaceuticals (Basel)."; date: "2020-12-21"; volume: "13(12). 
            pii: ph13120481. doi"; pages: " 10.3390/ph13120481"
COMMENT     Sequence Label: pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG
FEATURES             Location/Qualifiers
     source          1..10492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        110..296
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             297..959
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             complement(1117..1488)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELVRINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            1521..1630
                     /note="incP origin of transfer"
     CDS             2545..2940
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             join(6050..6052,6053..6055,6056..6568)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(6095..6116)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6356..6375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6703..6724
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6764..6781
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6782..6799
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6800..6823
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      complement(8149..8737)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8229..8248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8828..9619)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    join(10098..10492,1..109)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta
       61 ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc tagtacactc
      121 tatatttttt tatgcctcgg taatgatttt catttttttt ttccacctag cggatgactc
      181 tttttttttc ttagcgattg gcattatcac ataatgaatt atacattata taaagtaatg
      241 tgatttcttc gaagaatata ctaaaaaatg agcaggcaag ataaacgaag gcaaagatga
      301 cagagcagaa agccctagta aagcgtatta caaatgaaac caagattcag attgcgatct
      361 ctttaaaggg tggtccccta gcgatagagc actcgatctt cccagaaaaa gaggcagaag
      421 cagtagcaga acaggccaca caatcgcaag tgattaacgt ccacacaggt atagggtttc
      481 tggaccatat gatacatgct ctggccaagc attccggctg gtcgctaatc gttgagtgca
      541 ttggtgactt acacatagac gaccatcaca ccactgaaga ctgcgggatt gctctcggtc
      601 aagcttttaa agaggcccta ggggccgtgc gtggagtaaa aaggtttgga tcaggatttg
      661 cgcctttgga tgaggcactt tccagagcgg tggtagatct ttcgaacagg ccgtacgcag
      721 ttgtcgaact tggtttgcaa agggagaaag taggagatct ctcttgcgag atgatcccgc
      781 attttcttga aagctttgca gaggctagca gaattaccct ccacgttgat tgtctgcgag
      841 gcaagaatga tcatcaccgt agtgagagtg cgttcaaggc tcttgcggtt gccataagag
      901 aagccacctc gcccaatggt accaacgatg ttccctccac caaaggtgtt cttatgtagt
      961 tttacacagg agtctggact tgaccaatgg atcgtcttgc cttgctcgtc ggtgatgtac
     1021 ttcaccagct ccgcgaagtc gcccttcttg atggagcgca tggggacgtg cttggcaatc
     1081 acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat ggctctgccc tcgggcggac
     1141 cacgcccatc atgaccttgc caagctcgtc ctgcttctct tcgatcttcg ccagcagggc
     1201 gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag
     1261 caggccgccc aggcggccca ggtcgccatt gatgcggacc agctcgcgga cgtgctcata
     1321 gtccacgacg cccgtgattt tgtagccctg gccgacggcc agcaggtagg ccgacaggct
     1381 catgccggcc gccgccgcct tttcctcaat cgcccttcgt tcgtctggaa ggcagtacac
     1441 cttgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc
     1501 atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
     1561 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
     1621 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
     1681 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
     1741 gaagcagggt tatgcagcgg aagatcgcta gcaaggagtc cattttgaca tgtttcctag
     1801 ctagaaggaa cagctattca tatgaatttt ccgaggtcac gctgtgatgg atcgattcgt
     1861 tcccgtcaca gacgcaagca gcgaggtgat ggctggacgg gcacgcgtca caatttagaa
     1921 caacccggac tcttgtgacg cgagctccgg gtgcggaaaa ccctacgggg tgaaaggagc
     1981 cgcagcttcc acccatttac ttgactgata acccagtaca cacaacgcta ccgtgacgga
     2041 aatgccgcgc gctgtgaagc cgtggtgaat ctttcgctgc tgctcgttct ctccacacac
     2101 agaattgcac ggcttgacga ttagcaaaaa gcagaagaag cagtgtcaag atggtgagtg
     2161 tcttgagtac aaaagtagcg cacggtagtg cagaatacgg ctgtgtagac tatgcggcgt
     2221 cggagtagcc gagatcccgt aaaaaacggt aggaatcgga ggaatctcaa tgagtgatcg
     2281 tgctgcgtac gccgcttccg ttggggacca tacgatcgcg tagttcttgt tagcaacgac
     2341 atgcctttac gtcgcgaagg tctttcaagt aactaaaaca acggaatatg tcaagattac
     2401 ctcgatttga tggattcgaa tgaggatcgg gatcaggagt tctattgaca ctagttgatt
     2461 tcctcaatgc gaatgccgct caatgtcccc ctttctcatc tatgactgtt tacttccctt
     2521 aggtgaactt tacagttgaa cagaatggcc aagcctttgt ctcaagaaga atccaccctc
     2581 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     2641 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     2701 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     2761 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     2821 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     2881 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     2941 tcaggttaat gataggctta cagaaaaaca gactcatagg gtacttcgaa cataagaaaa
     3001 gattcattac atgacggatt tcagtttcgt attcgtatag gtttattgag ttgatgaaca
     3061 tggcggtcag atatgccaat cgcttttctt ggtacgagtt tgctaggcgc tatacaggag
     3121 tccaaacggt acgtcagatc ccacgtcact accgcacccg gttattccag tgcgtaagct
     3181 cgtagtggaa gaacaggctg cggtagtgac gtggaatttg acactttcca atcttcaaga
     3241 cctctagcca gcaaagactt cttgaatctg caaggatatt ctatttagat ataagtatat
     3301 acttgacagt gaggggggtc gtgaccgtct ctcgctggga tagagaagat ggcaaccatt
     3361 gaattgagaa ctcgaaactt gagcttggtt ggatcttgat cattctgaat ttgcaggata
     3421 ggttaacagt aaaagtcaat cttgcgctcg ccatctacca gaaagcacgt gggtgcccag
     3481 atcctcccaa cgagcgaacg gacggatgct agtcgggggg atctctgtgg gccattcaaa
     3541 cgaaaggcag atggcatcgt tccaagatct attggtcatt gggcttctca tacagtcatt
     3601 gacaccacga ttgaagacaa atttttgttg acagtgaaat cgttgcggac gcgctccgat
     3661 tcaaaagttt ccctactcgg tcgcgtcaac cgaccttccc ctcgtgagtt tacagcttga
     3721 tacgccctat ttggcttcct atgtaaagtc tccagtgatc atattaaagt cattggtcat
     3781 gacactctcc ttgttcaaaa tttggtacca tacaatgcgc ggagcagtca gttctcattt
     3841 ccagcgatcg ttaaaacaat tagagaaatg ccttacttct ctctttatat ctgtattact
     3901 cagagtcagt gagtagtacg aatttccaac gtcctagctc caatttatgg tttcaaacaa
     3961 tccgaattcg ttggtccaca gtcaaggaat tcgcaagaat ccctatttca ctaggcgtgc
     4021 actatggggt cgcttttgag gttctcattt gcggcgatcg ttaaaacgat tacagaaatg
     4081 ctacacttcg ctctttatat atattactca ttgagcatca ggaatgtcca atgtcgtaac
     4141 tccgattcat ggtttcaaac aatccgaatt cgttggtccg aggaatccgc aaaaagtaat
     4201 tgtagtctat gtacgaccga gtcgctggtg agggtttttt gtcgttggat tgctcgagcg
     4261 aattctccga gattgtacag aaagctcaac tttcggaagg catctatgtg gtccaaccaa
     4321 tctattcaaa tatatcaatt gttaagagca gagttcgatc ctcactatgt ggaggggcat
     4381 ggtgagatta cggagtgccg agacataaga gctccctgca atcaagttga tggacatgct
     4441 tcgaaaatgc catgactgag aacgagaacg cccagatgct ccgcggcacg agaactcgat
     4501 gaacacgaac cgaatccaac gccaaaaacg cgttgtccaa aacattgcaa ctctctctga
     4561 taaaaaccaa aacgccgcaa attgcaccag cttttttggt tgtcctacgc ctctttccgc
     4621 accaccaatg gactctattt ccaccaagac cagcgcgtcc aacactcttt ccgctcggca
     4681 tcctcccgcc ccgtcggcac ccgtatgcag tgatccctcc agtttcacac caatccatac
     4741 cagcaagacc aacattacga cactagataa cgctgccatc tgtcaactgg tgctcaccaa
     4801 tcagatcaag gaccacgaac tcgaaaaacg tctcgacgcg caccgcgccg tgcaggtccg
     4861 ccgtttagtc gtcgcccaca aactcgacac cttggaacac atcaacgcgc acgctctcga
     4921 caatctgccc tcggaacctt ccctcgacta cacccgcgta cacggcgcca attgcgaaat
     4981 cgttgtgggc tacgtacccc tcccggtggg actcgtcggt cccctcacgg tcaatggcga
     5041 aaccgtctac gtgcccatgg ccaccaccga aggatgtctc gtggcctcca cgaaccgcgg
     5101 tgccaaggcc atcacggccg ggggtggagc gaccgccgtc ctcctccggg acggtattac
     5161 tcgggcgccc tgcgtacgca tgccctcggc agcgcaagcc gctcatctca aattgtggtg
     5221 tgaaacgccc caacacttta gtacccttaa acgagccttt gaaagtacca cctcgttcgg
     5281 gaaacttctc aagtgcgccc ccacagtcgc tggacgtaac gtgtatctgc gcttgacctg
     5341 cttttctgga gacgccatgg gcatgaacat ggtttccaag ggcagtttgg ccgtcataga
     5401 aacccttcaa cgagaatttc cggaactcgt cctcgtcgcc ttgtctggca acatgtgcac
     5461 cgataagaaa gccgccgcta ccaactggct ggaaggacga ggaaaatcga tcgtcgtgga
     5521 agcaaccatt cccaaggacg tcgtcacaaa caccctcaag actacggtcc acagtatggt
     5581 acaaactaat ttgcacaaga atttgatcgg ttcggccatg gccggagctt tgggcggatt
     5641 taacgcccac gcctccaaca ttgttaccgc ggtctttttg gccacgggac aggaccccgc
     5701 gcagaacgtg gagtccagta attgcattac cctgttggaa gaaacggaag aaggcgactt
     5761 gtggatttcc tgcaccatgc ccagcattga ggtcggtacg gtcggtggcg gaacttcctt
     5821 acccgcccaa gcggcctgtt tgcaggccat gggtgtccgg ggcggaggcg ccactcccgg
     5881 cgcccacgcc caaaaactgg cacaaatcgt cgcgtccgct actttggccg gcgaactttc
     5941 gctgctggcc gcactcgccg cgaataccct cgtacaggcc cacatgcaac acaaccgcaa
     6001 acccgccgcc aagtcaggta acggagatct gggtggctcc ggcggccgga tggtgagcaa
     6061 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     6121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     6181 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     6241 cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     6301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     6361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     6421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     6481 caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
     6541 caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca
     6601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     6661 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6721 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg gcgctggtcc ctcgcggtag
     6781 ccaccaccac catcaccacg actacaagga tgatgacgat aagtgagctt ttcgatcgac
     6841 gagcttacct tcacggccac cgttcccgac cccgacgttg ttcaggcgat cattacgggt
     6901 ggcagcgccg ctccgggagc caccggtttg tacggcattg ccaccgcaga tgccgagtgc
     6961 ccggccaacg agtgcatcca tgtggacgtg attgtgtacg ccattttcgc gaatgaagcc
     7021 acgaccaact tgattgtgga aatcagcggc gacgcggtca tgtctgtggg acgtactgcc
     7081 cgcaagttgc gtgcgaaagt ccagcccaca cgcgagttgc aggagacgct ccagatgcaa
     7141 gcctttcgta acgagattgt cctcccccct ttgcctggtt cgggggccac gttggccaac
     7201 gtgtgcgcgg cggcggtgcc cttcgccgtg gccgtggtgg ccggtgcgct cgctctttaa
     7261 aaagtgagag tttgtgtgtg tgaaagacgc gtcgcgagac gctccttatt tccgtcggca
     7321 tgtgtcttgt cgtggtccta tgtgatcccc gggtcggttc acgggagttg ccgaagtaga
     7381 gaataagaat tatataactg tacagagata cagacatgtg ctgctattac gtccaacagt
     7441 aggtatacac ctcccgggca atagagtggg ccacatccat acacacacac ccgagttggt
     7501 agattgattt cggttcgtcg aatgccaaga atgtgcttcc tgaagaaaat ttgctccttc
     7561 ccactccgtc cgtacgacca tccgcccgtg tacagcgcgg cagaagtaga aacgagtacc
     7621 aaggctctgt gtgtgtgtgt gtaccgtttg gacgcgtcgg caaaggtacg gcagtcctcc
     7681 gggtgtgtcc cgtactcgtg gtaccaacga atctgcacaa ttgtgccgtt cggaggacac
     7741 gccttcccgg atgcatgcat ccggaagacc gtcgatgcga ttcgctacag aggaggggaa
     7801 caaaggattg tgcagtgcca tacgacaagt tcgcctcaat cgccaaaccc cttgactgtg
     7861 cttcccgagc ggaaacattt agattggcta ctacaaccgt tgctctccgg gcggataaga
     7921 gaataaatct ggattcggca gagtagtcgg ccttatcatc agttagatgt tgataatccc
     7981 gttgggcagg tcacacgcta ggtatacttg agaccacccc aggatacata gattaccaca
     8041 actccgagcc cttccaccca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8101 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8161 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8221 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8281 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8341 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8401 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8461 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8521 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8581 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8641 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8701 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8761 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
     8821 gtgattatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
     8881 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     8941 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
     9001 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
     9061 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
     9121 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
     9181 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
     9241 tcgtggctgg ctcgaagata ccagcaagaa tgtcattgcg ctgccattct ccaaattgca
     9301 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
     9361 ctacagcgcg gagaatctca ctctctccag gggaagccga agtttccaaa aggtcgttga
     9421 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
     9481 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
     9541 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
     9601 cgatcaccgc ttccctcatg cgaaacgatc ctcatcctgt ctcttgatca gatattgatc
     9661 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     9721 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctaag cttgcatgcc
     9781 tgcaagtcga ctctagagga gcggtgatca caggcagcaa cgctctgtca tcgttacaat
     9841 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
     9901 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
     9961 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctcattac
    10021 tcgcatccat tctcaggctg tctcgtctcg tctccgaggt ctcaggagaa tgaggagcta
    10081 gctcgtcttc agcgagcatc acgtgctata aaaataatta taatttaaat tttttaatat
    10141 aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt
    10201 ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc
    10261 gtcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca
    10321 cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct
    10381 gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc
    10441 tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt ac
//
