LOCUS       sequence-296999-        3819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constutitive expression of LuxR protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Barbier I, Perez-Carrasco R, Schaerli Y
  TITLE     Controlling spatiotemporal pattern formation in a concentration 
            gradient with a synthetic toggle switch.
  JOURNAL   Mol Syst Biol. 2020 Jun;16(6):e9361. doi: 10.15252/msb.20199361.
  PUBMED    32529808
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2020-06"; volume: "16(6):e9361. doi"; pages: " 
            10.15252/msb.20199361"
COMMENT     Sequence Label: pCDF_LuxR
FEATURES             Location/Qualifiers
     source          1..3819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(104..195)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1208..1225
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1209..1225
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2727..2745)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2741..2788
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2797..2883
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2975..3002
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(3131..3819,1..103))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDDGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
       61 ctgatagttg agtcgatact tcggcgatca ccgcttccct catactcttc ctttttcaat
      121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
      181 agaaaaataa acaaatagct agctcactcg gtcgctacgc tccgggcgtg agactgcggc
      241 gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag gggactaaca
      301 tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc cataggctcc gccctcctgc
      361 cagagttcac ataaacagac gcttttccgg tgcatctgtg ggagccgtga ggctcaacca
      421 tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg tttccggcgg
      481 gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac ctgttccgcc
      541 tttctccctt acgggaagtg tggcgctttc tcatagctca cacactggta tctcggctcg
      601 gtgtaggtcg tttgctccaa gctgggctgt aagcaagaac tccccgttca gcccgactgc
      661 tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgg taaaacgcca
      721 ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt
      781 tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc
      841 tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa
      901 ccacctcccc aggtggtttt ttcgaaggtt aagtcagttg gggaactgct taaccttcga
      961 tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg cgcagaaaaa
     1021 aaggatctca agaagatcct ttgatctttt ctactgaacc gctctagatt tcagtgcaat
     1081 ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg taattctcat
     1141 gttagtcatg ccccgcgccc accggaagga gctgactggg ttgctcctag gatgcagtga
     1201 ctcgagttgt aaaacgacgg ccagtcaatt gttgcctcag gcattagaaa tagcgcgtta
     1261 ttgtacacgg attaatcgca ctacaaaaca ggatcacaaa catcctccgc aaacaagtat
     1321 tgcagagtcc ctttgtgatc gctttcacgg agcataaaaa gggttatcca aaggtcattg
     1381 caccaacatg gtgcttaatg tttccattga agcactatat tggtgcaaca ttcacatcgt
     1441 ggtgcagccc ttttgcacga tggtgcgcat gataacgcct tttaggggca atttaaaagt
     1501 tggcacagat ttcgctttat cttttttacg gcgacacggc caaaataatt gcagatttcg
     1561 ttaccacgac gaccatgacc aatccaggag agttaaagtt caattggaat tcatttcacc
     1621 caaggagctc gttgagttac ctgcagcgat tgtactagtt gcggccgcct gcgcaccacc
     1681 tgcaggacct cagcaatcgc ttggaccagc tttcttaagg gtaccacctg tacgatccta
     1741 caggtgctta tgttaagtaa ttgtattccc agcgatacaa tagtgtgaca aaaatccaat
     1801 ttattagaat caaatgtcaa tccattaccg ttttaatgat atataacacg caaaacttgc
     1861 gacaaacaat aggtaaggat aaagagatgg gtatgaaaaa catcaacgcc gacgatacgt
     1921 accgcatcat caacaaaatc aaagcttgtc gctcaaacaa cgacatcaac cagtgcctga
     1981 gcgatatgac caaaatggtt cattgcgaat attacctgct ggcgattatc tatccgcaca
     2041 gtatggtcaa atcagatatt tcgatcctgg acaactaccc gaaaaaatgg cgtcagtatt
     2101 acgatgacgc caatctgatt aaatatgatc cgatcgtgga ctacagtaac tccaatcact
     2161 ccccgattaa ctggaatatc tttgaaaaca atgcggtcaa caaaaaatct ccgaacgtga
     2221 tcaaagaagc caaaaccagc ggcctgatta cgggtttttc gttcccgatc cataccgcaa
     2281 acaatggctt tggtatgctg agcttcgctc actctgaaaa agataactat attgacagcc
     2341 tgttcctgca tgcatgcatg aatattccgc tgatcgtgcc gtctctggtt gataactacc
     2401 gtaaaatcaa catcgctaac aacaaaagta acaatgacct gaccaaacgc gaaaaagaat
     2461 gcctggcgtg ggcctgtgaa ggcaaaagct cttgggatat tagtaaaatc ctgggttgtt
     2521 ccgaacgtac cgttacgttt catctgacga acgcgcaaat gaaactgaac accacgaatc
     2581 gctgccaatc catctccaaa gcaatcctga ccggcgctat cgactgtccg tacttcaaaa
     2641 attaataagg atccggcgcc gctcattcgc taatcgccac gacgcgtagt cgacccgcca
     2701 attcccatgg ggcgcgcctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     2761 ctctaaacgg gtcttgaggg gttttttgcc aggcatcaaa taaaacgaaa ggctcagtcg
     2821 gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     2881 aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg gcgggcagga
     2941 cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac ggatggcctt
     3001 tttgcgtttc agatctaccg gtaaaccagc aatagacata agcggctatt taacgaccct
     3061 gccctgaacc gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg cttgaacgaa
     3121 ttgttagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     3181 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     3241 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     3301 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     3361 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     3421 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     3481 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     3541 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     3601 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     3661 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     3721 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     3781 tatcactgtg tggcttcagg ccgtcatcca ctgcggagc
//
