LOCUS       sequence-297103-       10977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo (without an ER export 
            signal) for Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 10977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C...
FEATURES             Location/Qualifiers
     source          1..10977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    285..309
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(395..647)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(657..1208)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1214..1594)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2590..2935
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2878..2898
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      4031..4738
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(4763..4780)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4763..4779)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4772..4794)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4982..5006
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6307..6936
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7365..8438
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8504..8698
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8934..8956)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9042..9182
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9097..9116
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9197..9214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9368..9956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9448..9467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10043..10837)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
       61 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
      121 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
      181 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
      241 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
      301 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
      361 tgaattaacg ccgaattaat tcgagctcgg atctgataat ttatttgaaa attcataaga
      421 aaagcaaacg ttacatgaat tgatgaaaca atacaaagac agataaagcc acgcacattt
      481 aggatattgg ccgagattac tgaatattga gtaagatcac ggaatttctg acaggagcat
      541 gtcttcaatt cagcccaaat ggcagttgaa atactcaaac cgccccatat gcaggagcgg
      601 atcattcatt gtttgtttgg ttgcctttgc caacatggga gtccaagatt ctgcagtcaa
      661 atctcggtga cgggcaggac cggacggggc ggtaccggca ggctgaagtc cagctgccag
      721 aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat gccgcggggg
      781 gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg
      841 accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta gagcgtggag
      901 cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc cgtccagtcg
      961 taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc gccgtccacc
     1021 tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt ccactcctgc
     1081 ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg
     1141 gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct
     1201 gggctcatcg attcgatttg gtgtatcgag attggttatg aaattcagat gctagtgtaa
     1261 tgtattggta atttgggaag atataatagg aagcaaggct atttatccat ttctgaaaag
     1321 gcgaaatggc gtcaccgcga gcgtcacgcg cattccgttc ttgctgtaaa gcgttgtttg
     1381 gtacactttt gactagcgag gcttggcgtg tcagcgtatc tattcaaaag tcgttaatgg
     1441 ctgcggatca agaaaaagtt ggaatagaaa cagaataccc gcgaaattca ggcccggttg
     1501 ccatgtccta cacgccgaaa taaacgacca aattagtaga aaaataaaaa ctgactcgga
     1561 tacttacgtc acgtcttgcg cactgatttg aaaaatctca gaattccaat cccacaaaaa
     1621 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
     1681 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
     1741 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
     1801 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
     1861 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
     1921 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
     1981 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
     2041 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
     2101 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
     2161 gcaggtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
     2221 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
     2281 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
     2341 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
     2401 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tgaagactgg
     2461 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
     2521 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagacca
     2581 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     2641 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     2701 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     2761 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     2821 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     2881 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     2941 gacatgaaag ccttcacact cgctctcttc ttagctcttt ccctctatct cctgcccaat
     3001 ccagccgaca gcacagaaaa cgttataaaa ccctttatgc gttttaaagt ccatatggag
     3061 gggagcgtca atggacacga atttgagatt gagggtgaag gagagggcaa gccttacgag
     3121 ggaacccaaa ctgcgaagct acaggtgacc aagggcggac ccctcccatt tgcttgggac
     3181 attctgtctc cgcaattcca atatggcagt aaagtgtatg tcaaacaccc ggccgacata
     3241 ccggactaca agaagttatc atttccggaa ggtttcaagt gggagagagt catgaatttt
     3301 gaagacgggg gggtagtaac agtgactcag gacagcagtc ttcaagacgg tacgttcatc
     3361 tatcatgtca aatttatcgg agtcaacttc ccttctgacg gaccggttat gcagaagaag
     3421 actctgggct gggaaccgag tactgagcga ctgtaccctc gtgatggagt actgaaaggt
     3481 gagatacata aagcactaaa gctcaagggc ggcggtcatt atctcgtgga atttaagagt
     3541 atttacatgg ctaaaaaacc cgtaaagcta cctggatatt attatgttga ttctaaacta
     3601 gatattacga gccataacga agactatacc gtcgtcgagc agtatgagag agcagaggcg
     3661 cgacatcatt tattccaggg ctcaagcaga ggagttcagg tcgaaactat tagccccgga
     3721 gatggccgaa cctttccgaa aaggggccag actgtagttg tacactatac gggaatgctc
     3781 gaggacggaa agaagttaga cagctcccga gacagaaaca aaccgttcaa gttcatgctg
     3841 ggtaagcaag aagtaattcg aggctgggaa gaaggcgtag cccaaatgag cgtaggacaa
     3901 cgagccaaac ttacgatttc accagattac gcctatgggg ctactggaca tcccggagtg
     3961 atacctcctc atgcaacgct cgtttttgat gttgagttat tgaagttaga gtaaactagt
     4021 ccctagagtc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt
     4081 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg
     4141 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt
     4201 ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca
     4261 atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca
     4321 attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa
     4381 aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct agtatctacg
     4441 acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc ccgccggcgc
     4501 gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa agttacgggc
     4561 accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc gagaattatg
     4621 cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct tgacagtgac
     4681 gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg ctcagcagga
     4741 cctgcaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4801 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4861 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct
     4921 agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt
     4981 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
     5041 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
     5101 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     5161 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     5221 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     5281 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     5341 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     5401 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     5461 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     5521 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     5581 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     5641 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     5701 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     5761 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     5821 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     5881 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     5941 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     6001 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     6061 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
     6121 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     6181 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     6241 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     6301 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     6361 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     6421 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     6481 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     6541 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     6601 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     6661 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     6721 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     6781 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     6841 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     6901 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     6961 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     7021 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     7081 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     7141 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     7201 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     7261 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc
     7321 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     7381 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     7441 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     7501 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     7561 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     7621 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     7681 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     7741 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     7801 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     7861 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     7921 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     7981 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
     8041 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
     8101 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
     8161 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
     8221 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
     8281 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
     8341 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
     8401 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
     8461 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
     8521 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
     8581 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
     8641 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
     8701 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
     8761 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
     8821 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
     8881 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     8941 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     9001 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     9061 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     9121 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     9181 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9241 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9301 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9781 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     9841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10021 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt
    10081 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
    10141 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
    10201 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
    10261 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
    10321 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
    10381 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
    10441 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
    10501 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
    10561 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
    10621 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
    10681 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
    10741 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
    10801 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
    10861 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
    10921 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaa
//
