LOCUS       sequence-297144-       11953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with tapasin CT 
            deltaALAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11953)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 11953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-tapasinCT deltaALAS
FEATURES             Location/Qualifiers
     source          1..11953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1586..1720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1610..1629)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1664..1683
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1862..1884)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2470..4259
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4565..6490)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6925..6943)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7011..7115
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7116..7184,7185..7976)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7334..7353)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8147..8735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8636..8655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9352..9497
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9537..10562
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9537..10562
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10583..10602)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10627..10675
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11328..11707
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11708..11911
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11862..11882
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11908..11932
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatggccgt catggcgccc cgaaccctcg tcctgctact ctcgggggcc
       61 ctggccctga cccagacctg ggcgggctcc gactacaagg acgatgatga caagggagga
      121 tccaagcccc acccagcaga ggggcagtgg cgggcagtgg acgtggtcct agactgtttc
      181 ctggtgaagg acggtgcgca ccgtggaagt gaggacaggg caagggcctc ccttgtgctg
      241 aagcaggtgc cagtgctgga cgatggctcc ctggaggact tcaccgattt ccaagggggc
      301 acactggccc aagatgaccc acctattatc tttgaggcct cagtggacct ggtccagatt
      361 ccccaggccg aggccttgct ccatgctgac tgcagtggga aggaggtgac ctgtgagatc
      421 tcccgctact ttctccagat gacagagacc actgttaaga cagcagcttg gttcatggcc
      481 aacgtgcagg tctctggagg gggacctagc atctccttgg tgatgaagac tcccagggtc
      541 gccaagaatg aggtgctctg gcacccaacg ctgaacttgc cactgagccc ccaggggact
      601 gtgcgaactg cagtggagtt ccaggtgatg acacagaccc aatccctgag cttcctgctg
      661 gggtcctcag cctccttgga ctgtggcttc tccatggcac cgggcttgga cctcatcagt
      721 gtggagtggc gactgcagca caagggcagg ggtcagttgg tgtacagctg gaccgcaggg
      781 caggggcagg ctgtgcggaa gggcgctacc ctggagcctg cacaactggg catggccagg
      841 gatgcctccc tcaccctgcc cggcctcact atacaggacg aggggaccta catttgccag
      901 atcaccacct ctctgtaccg agctcagcag atcatccagc tcaacatcca agcttcccct
      961 aaagtacgac tgagcttggc aaacgaagct ctgctgccca ccctcatctg cgacattgct
     1021 ggctattacc ctctggatgt ggtggtgacg tggacccgag aggagctggg tggatcccca
     1081 gcccaagtct ctggtgcctc cttctccagc ctcaggcaaa gcgtggcagg cacctacagc
     1141 atctcctcct ctctcaccgc agaacctggc tctgcaggtg ccacttacac ctgccaggtc
     1201 acacacatct ctctggagga gccccttggg gccagcaccc aggttgtccc accagagcgg
     1261 agatcagggc cctcccttga ggacagcgta ggccttttcc tgtctgcctt tcttctgctt
     1321 gggctcttca aggcactggg ctgggctgct gtctacctgt ccacctgcaa ggattcaaag
     1381 aaggtacagt gctccacctc tctgtatctt tcccttgtca ctttatctcc tcatcctatc
     1441 tcaaaaccca tggagggagg ctgctggtgt ggtaggcaga acctaggctt ggaattcaca
     1501 ctgatctggg ttaaaacctg gcactatatc ctaactgtag gactctttga gcatgctact
     1561 taatgactcg aggccggcaa ggccggatcc agacatgata agatacattg atgagtttgg
     1621 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1681 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1741 ttttatgttt caggttcagg gggaggtggg gaggtttttt aaagcaagta aaacctctac
     1801 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     1861 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     1921 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     1981 ccggtcgact ctagagcccc gccgccggac gaactaaacc tgactacggc atctctgccc
     2041 cttcttcgct ggtacgagga gcgcttttgt tttgtattcg gggcagtgca tgtaatccct
     2101 tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac
     2161 caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg
     2221 ccaacactga gtggctttca tcctggagca gactttgcat gctgtggact gcaacacaac
     2281 attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc
     2341 tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt
     2401 agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg
     2461 ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc
     2521 ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa
     2581 aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat
     2641 ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat
     2701 caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta
     2761 gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa
     2821 ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc
     2881 tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa
     2941 acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg
     3001 atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata
     3061 ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac
     3121 tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc
     3181 tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa
     3241 gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc
     3301 cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa
     3361 ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca
     3421 taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca
     3481 cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg
     3541 agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca
     3601 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     3661 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     3721 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     3781 tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta
     3841 tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg
     3901 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg
     3961 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4021 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4081 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     4141 ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc
     4201 tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc
     4261 atgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt
     4321 ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg
     4381 gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga ggacgaaaat
     4441 ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta ctggggattt
     4501 attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct cctaacaagt
     4561 tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct ccgtcatctc
     4621 cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga ggcaaatcta
     4681 ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg ggctttgtca
     4741 taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga
     4801 ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg ggtccagggg
     4861 ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata gcaagggcag
     4921 ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac acaccggcga
     4981 cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga gctcttaaac
     5041 cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc tttccaaacc
     5101 accctccttt tttgcgcctg cctccatcac cctgaccccg gggtccagtg cttgggcctt
     5161 ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg gggcacgtca ggctcaccat
     5221 ctgggccacc ttcttggtgg tattcaaaat aatcggcttc ccctacaggg tggaaaaatg
     5281 gccttctacc tggagggggc ctgcgcggtg gagacccgga tgatgatgac tgactactgg
     5341 gactcctggg cctcttttct ccacgtccac gacctctccc cctggctctt tcacgacttc
     5401 cccccctggc tctttcacgt cctctacccc ggcggcctcc actacctcct cgaccccggc
     5461 ctccactacc tcctcgaccc cggcctccac tgcctcctcg accccggcct ccacctcctg
     5521 ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc
     5581 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     5641 cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     5701 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc
     5761 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg
     5821 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct gcccctcctg
     5881 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     5941 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     6001 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
     6061 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
     6121 cccctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
     6181 ctgcccctcc tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc
     6241 ctttgcagcc aatgcaactt ggacgttttt ggggtctccg gacaccatct ctatgtcttg
     6301 gccctgatcc tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc
     6361 cccgtcctcg tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt
     6421 ctggtccaga tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct
     6481 cgtcagacat gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt
     6541 gaatacaggg agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca
     6601 tggtcgctgt cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc
     6661 tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc
     6721 tgagcctcaa gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg
     6781 gattctcggg acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca
     6841 acggaagaaa agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac
     6901 atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6961 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7021 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7081 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7141 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7201 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7261 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7321 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     7381 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7441 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7501 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7561 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7621 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     7681 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7741 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7801 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7861 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7921 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7981 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8041 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8101 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8161 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8221 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8281 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8341 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8401 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8461 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8521 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8581 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8641 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8701 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8761 ggttcctggc cttttgctgc gccgcgtgcg gctgctggag atggcggacg cgatggatat
     8821 gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
     8881 tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc
     8941 cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca
     9001 atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc
     9061 ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga
     9121 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
     9181 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
     9241 acgtagccca gcgcgtcggc cgccatgccc tgcttcatcc ccgtggcccg ttgctcgcgt
     9301 ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa
     9361 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
     9421 ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
     9481 tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga tcccgggcaa tgagatatga
     9541 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     9601 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     9661 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     9721 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     9781 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     9841 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     9901 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     9961 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
    10021 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
    10081 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
    10141 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
    10201 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
    10261 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
    10321 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
    10381 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
    10441 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
    10501 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
    10561 aggggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
    10621 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
    10681 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
    10741 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
    10801 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag
    10861 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg
    10921 cgtggggtct ggtccacgac tggactgagc agacagaccc atggtttttg gatggcctgg
    10981 gcatggaccg catgtactgg cgcgacacga acaccgggcg tctgtggctg ccaaacaccc
    11041 ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca agctagtcga ccaattctca
    11101 tgtttgacag cttatcatcg cagatccggg caacgttgtt gcattgctgc aggcgcagaa
    11161 ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat
    11221 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
    11281 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt
    11341 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    11401 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    11461 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    11521 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    11581 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    11641 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    11701 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
    11761 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
    11821 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
    11881 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tctctagaag
    11941 ctgggtacca gct
//
