LOCUS       sequence-297297-        4419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4419)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133213
FEATURES             Location/Qualifiers
     source          1..4419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             188..208
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             209..235
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    288..409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(299..318)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     353..372
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      524..570
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      600..736
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    781..852
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1078..1666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1158..1177)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2412..2480,2481..3272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2630..2649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3285..3421
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3573..3644
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3649..3695)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3713..3761
                     /note="synthetic polyadenylation signal"
     enhancer        3842..4145
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4147..4349
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4300..4320
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 caccatgttc cacttggtgg attttcaggt cacgatcgca gaaatactgc taattatcat
       61 gcgaaccttc aaggttagca tttggaacct ggactacatc attaatctca tcataaaaaa
      121 ccttagtaag tctttaactg agaataaata ttcccaactg gatgaggaac agccaatgga
      181 gattgacgaa aacctttact ttcagtcgta cccctatgac gtaccagatt atgcgtaagc
      241 tagcttgact gactgagata cagcgtacct tcagctcaca gacatgataa gatacattga
      301 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      361 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
      421 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
      481 aaacctctac aaatgtggta ttggcccatc tctatcggta tcgtagcata accccttggg
      541 gcctctaaac gggtcttgag gggttttttg tgcccctcgg gccggattgc tatctaccgg
      601 cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc cacatatgcc
      661 agattcagca acggatacgg cttccccaac ttgcccactt ccatacgtgt cctccttacc
      721 agaaatttat ccttaaggtc gtcagctatc ctgcaggcga tctctcgatt tcgatcaaga
      781 cattccttta atggtctttt ctggacacca ctaggggtca gaagtagttc atcaaacttt
      841 cttccctccc taatctcatt ggttaccttg ggctatcgaa acttaattaa ccagtcaagt
      901 cagctacttg gcgagatcga cttgtctggg tttcgactac gctcagaatt gcgtcagtca
      961 agttcgatct ggtccttgct attgcacccg ttctccgatt acgagtttca tttaaatcat
     1021 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1081 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1141 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1201 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1261 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1321 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1381 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1441 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1501 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1561 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1621 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1681 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1741 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1801 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1861 acctatctca gcgatctgtc tatttcgttc atccatagtt gcatttaaat ttccgaactc
     1921 tccaaggccc tcgtcggaaa atcttcaaac ctttcgtccg atccatcttg caggctacct
     1981 ctcgaacgaa ctatcgcaag tctcttggcc ggccttgcgc cttggctatt gcttggcagc
     2041 gcctatcgcc aggtattact ccaatcccga atatccgaga tcgggatcac ccgagagaag
     2101 ttcaacctac atcctcaatc ccgatctatc cgagatccga ggaatatcga aatcggggcg
     2161 cgcctggtgt accgagaacg atcctctcag tgcgagtctc gacgatccat atcgttgctt
     2221 ggcagtcagc cagtcggaat ccagcttggg acccaggaag tccaatcgtc agatattgta
     2281 ctcaagcctg gtcacggcag cgtaccgatc tgtttaaacc tagatattga tagtctgatc
     2341 ggtcaacgta taatcgagtc ctagcttttg caaacatcta tcaagagaca ggatcagcag
     2401 gaggctttcg catgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2461 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2521 agttgggtgc gcgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2581 gttttcgccc cgaagaacgc tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2641 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2701 agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2761 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2821 tgacaacgat tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2881 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2941 acaccacgat gcctgtagca atggcaacaa ccttgcgtaa actattaact ggcgaactac
     3001 ttactctagc ttcccggcaa cagttgatag actggatgga ggcggataaa gttgcaggac
     3061 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3121 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3241 agataggtgc ctcactgatt aagcattggt aaccgattct aggtgcattg gcgcagaaaa
     3301 aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga
     3361 tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta
     3421 agatcccgaa tcgtttaaac tcgactctgg ctctatcgaa tctccgtcgt ttcgagctta
     3481 cgcgaacagc cgtggcgctc atttgctcgt cgggcatcga atctcgtcag ctatcgtcag
     3541 cttacctttt tggcagcgat cgcggctccc gacatcttgg accattagct ccacaggtat
     3601 cttcttccct ctagtggtca taacagcagc ttcagctacc tctcaattca aaaaacccct
     3661 caagacccgt ttagaggccc caaggggtta tgctatcaat cgttgcgtta cacacacaaa
     3721 aaaccaacac acatccatct tcgatggata gcgattttat tatctaactg ctgatcgagt
     3781 gtagccagat ctagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3841 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3901 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3961 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4021 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4081 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4141 ccatgctgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4201 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4261 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     4321 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc agatctttgt
     4381 cgatcctacc atccactcga cacacccgcc agcggccgc
//
