LOCUS       sequence-297601-        4224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4224)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136048
FEATURES             Location/Qualifiers
     source          1..4224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    93..214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(104..123)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     158..177
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      329..375
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      405..541
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    586..657
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(883..1471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(963..982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2217..2285,2286..3077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2435..2454)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3090..3226
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3378..3449
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3454..3500)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3518..3566
                     /note="synthetic polyadenylation signal"
     enhancer        3647..3950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3952..4154
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4105..4125
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      join(4223..4224,1..8)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caccatggct gatgcacaat cgtttttaaa cgggtttgcg taagctagct tgactgactg
       61 agatacagcg taccttcagc tcacagacat gataagatac attgatgagt ttggacaaac
      121 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      181 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      241 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      301 tggtattggc ccatctctat cggtatcgta gcataacccc ttggggcctc taaacgggtc
      361 ttgaggggtt ttttgtgccc ctcgggccgg attgctatct accggcattg gcgcagaaaa
      421 aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga
      481 tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta
      541 aggtcgtcag ctatcctgca ggcgatctct cgatttcgat caagacattc ctttaatggt
      601 cttttctgga caccactagg ggtcagaagt agttcatcaa actttcttcc ctccctaatc
      661 tcattggtta ccttgggcta tcgaaactta attaaccagt caagtcagct acttggcgag
      721 atcgacttgt ctgggtttcg actacgctca gaattgcgtc agtcaagttc gatctggtcc
      781 ttgctattgc acccgttctc cgattacgag tttcatttaa atcatgtgag caaaaggcca
      841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1681 ctgtctattt cgttcatcca tagttgcatt taaatttccg aactctccaa ggccctcgtc
     1741 ggaaaatctt caaacctttc gtccgatcca tcttgcaggc tacctctcga acgaactatc
     1801 gcaagtctct tggccggcct tgcgccttgg ctattgcttg gcagcgccta tcgccaggta
     1861 ttactccaat cccgaatatc cgagatcggg atcacccgag agaagttcaa cctacatcct
     1921 caatcccgat ctatccgaga tccgaggaat atcgaaatcg gggcgcgcct ggtgtaccga
     1981 gaacgatcct ctcagtgcga gtctcgacga tccatatcgt tgcttggcag tcagccagtc
     2041 ggaatccagc ttgggaccca ggaagtccaa tcgtcagata ttgtactcaa gcctggtcac
     2101 ggcagcgtac cgatctgttt aaacctagat attgatagtc tgatcggtca acgtataatc
     2161 gagtcctagc ttttgcaaac atctatcaag agacaggatc agcaggaggc tttcgcatga
     2221 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2281 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcgcgag
     2341 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2401 aacgctttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2461 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2521 agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2581 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgattggag
     2641 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2701 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2761 tagcaatggc aacaaccttg cgtaaactat taactggcga actacttact ctagcttccc
     2821 ggcaacagtt gatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2881 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2941 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3001 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3061 tgattaagca ttggtaaccg attctaggtg cattggcgca gaaaaaaatg cctgatgcga
     3121 cgctgcgcgt cttatactcc cacatatgcc agattcagca acggatacgg cttccccaac
     3181 ttgcccactt ccatacgtgt cctccttacc agaaatttat ccttaagatc ccgaatcgtt
     3241 taaactcgac tctggctcta tcgaatctcc gtcgtttcga gcttacgcga acagccgtgg
     3301 cgctcatttg ctcgtcgggc atcgaatctc gtcagctatc gtcagcttac ctttttggca
     3361 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
     3421 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
     3481 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
     3541 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
     3601 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     3661 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     3721 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     3781 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     3841 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     3901 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
     3961 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     4021 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     4081 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     4141 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
     4201 ctcgacacac ccgccagcgg ccgc
//
