LOCUS       sequence-297677-         231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 and gRNA expression plasmid for P. falciparum with yDHODH 
            selectable marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Nessel T, Beck JM, Rayatpisheh S, Jami-Alahmadi Y, Wohlschlegel JA, 
            Goldberg DE, Beck JR
  TITLE     EXP1 is required for organisation of EXP2 in the intraerythrocytic 
            malaria parasite vacuole.
  JOURNAL   Cell Microbiol. 2020 Jan 28:e13168. doi: 10.1111/cmi.13168.
  PUBMED    31990132
REFERENCE   2  (bases 1 to 231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Microbiol. 2020 Jan 28:e13168. doi: 10.1111/cmi.13168."
COMMENT     Sequence Label: pUF-Cas9-pre-sgRNA
FEATURES             Location/Qualifiers
     source          1..231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        122..197
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tatatatata tatatatata atattagagt aaccaaaatg cataattttt cctatatgca
       61 catatttcat attaagtata taatattcct aggaactcat cgctcgcgat gctgcccgac
      121 agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      181 tggcaccgag tcggtgcttt tttattattt cctataaaat aatatatata t
//
