LOCUS       sequence-298049-        3051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA 
            polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3051)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046531
FEATURES             Location/Qualifiers
     source          1..3051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2866..2883
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             2884..2901
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2946..2964)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tattacaact ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt
       61 tttaattaca aaatggctga tgcacaatcg tttttaaacc gggtttgcgg tgtaagtgca
      121 gcccgtctta caccgtgcgg cacaggcact agtactgatg tcgtatacag ggcttttgac
      181 atctacaatg ataaagtagc tggttttgct aaattcctaa aaactaattg ttgtcgcttc
      241 caagaaaagg acgaagatga caatttaatt gattcttact ttgtagttaa gagacacact
      301 ttctctaact accaacatga agaaacaatt tataatttac ttaaggattg tccagctgtt
      361 gctaaacatg acttctttaa gtttagaata gacggtgaca tggtaccaca tatatcacgt
      421 caacgtctta ctaaatacac aatggcagac ctcgtctatg ctttaaggca ttttgatgaa
      481 ggtaattgtg acacattaaa agaaatactt gtcacataca attgttgtga tgatgattat
      541 ttcaataaaa aggactggta tgattttgta gaaaacccag atatattacg cgtatacgcc
      601 aacttaggtg aacgtgtacg ccaagctttg ttaaaaacag tacaattctg tgatgccatg
      661 cgaaatgctg gtattgttgg tgtactgaca ttagataatc aagatctcaa tggtaactgg
      721 tatgatttcg gtgatttcat acaaaccacg ccaggtagtg gagttcctgt tgtagattct
      781 tattattcat tgttaatgcc tatattaacc ttgaccaggg ctttaactgc agagtcacat
      841 gttgacactg acttaacaaa gccttacatt aagtgggatt tgttaaaata tgacttcacg
      901 gaagagaggt taaaactctt tgaccgttat tttaaatatt gggatcagac ataccaccca
      961 aattgtgtta actgtttgga tgacagatgc attctgcatt gtgcaaactt taatgtttta
     1021 ttctctacag tgttcccacc tacaagtttt ggaccactag tgagaaaaat atttgttgat
     1081 ggtgttccat ttgtagtttc aactggatac cacttcagag agctaggtgt tgtacataat
     1141 caggatgtaa acttacatag ctctagactt agttttaagg aattacttgt gtatgctgct
     1201 gaccctgcta tgcacgctgc ttctggtaat ctattactag ataaacgcac tacgtgcttt
     1261 tcagtagctg cacttactaa caatgttgct tttcaaactg tcaaacccgg taattttaac
     1321 aaagacttct atgactttgc tgtgtctaag ggtttcttta aggaaggaag ttctgttgaa
     1381 ttaaaacact tcttctttgc tcaggatggt aatgctgcta tcagcgatta tgactactat
     1441 cgttataatc taccaacaat gtgtgatatc agacaactac tatttgtagt tgaagttgtt
     1501 gataagtact ttgattgtta cgatggtggc tgtattaatg ctaaccaagt catcgtcaac
     1561 aacctagaca aatcagctgg ttttccattt aataaatggg gtaaggctag actttattat
     1621 gattcaatga gttatgagga tcaagatgca cttttcgcat atacaaaacg taatgtcatc
     1681 cctactataa ctcaaatgaa tcttaagtat gccattagtg caaagaatag agctcgcacc
     1741 gtagctggtg tctctatctg tagtactatg accaatagac agtttcatca aaaattattg
     1801 aaatcaatag ccgccactag aggagctact gtagtaattg gaacaagcaa attctatggt
     1861 ggttggcaca acatgttaaa aactgtttat agtgatgtag aaaaccctca ccttatgggt
     1921 tgggattatc ctaaatgtga tagagccatg cctaacatgc ttagaattat ggcctcactt
     1981 gttcttgctc gcaaacatac aacgtgttgt agcttgtcac accgtttcta tagattagct
     2041 aatgagtgtg ctcaagtatt gagtgaaatg gtcatgtgtg gcggttcact atatgttaaa
     2101 ccaggtggaa cctcatcagg agatgccaca actgcttatg ctaatagtgt ttttaacatt
     2161 tgtcaagctg tcacggccaa tgttaatgca cttttatcta ctgatggtaa caaaattgcc
     2221 gataagtatg tccgcaattt acaacacaga ctttatgagt gtctctatag aaatagagat
     2281 gttgacacag actttgtgaa tgagttttac gcatatttgc gtaaacattt ctcaatgatg
     2341 atactctctg acgatgctgt tgtgtgtttc aatagcactt atgcatctca aggtctagtg
     2401 gctagcataa agaactttaa gtcagttctt tattatcaaa acaatgtttt tatgtctgaa
     2461 gcaaaatgtt ggactgagac tgaccttact aaaggacctc atgaattttg ctctcaacat
     2521 acaatgctag ttaaacaggg tgatgattat gtgtaccttc cttacccaga tccatcaaga
     2581 atcctagggg ccggctgttt tgtagatgat atcgtaaaaa cagatggtac acttatgatt
     2641 gaacggttcg tgtctttagc tatagatgct tacccactta ctaaacatcc taatcaggag
     2701 tatgctgatg tctttcattt gtacttacaa tacataagaa agctacatga tgagttaaca
     2761 ggacacatgt tagacatgta ttctgttatg cttactaatg ataacacttc aaggtattgg
     2821 gaacctgagt tttatgaggc tatgtacaca ccgcatacag tcttactcgt gccgagaggt
     2881 tctcaccatc atcaccatca ttaaacaggc cccttttcct ttgtcgatat catgtaatta
     2941 gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga aaaggaagga
     3001 gttagacaac ctgaagtcta ggtccctatt tatttttttt aatagttatg t
//
