LOCUS       sequence-29808-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Beskow A, Grimberg KB, Bott LC, Salomons FA, Dantuma NP, Young P
  TITLE     A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal
            degradation.
  JOURNAL   J Mol Biol. 2009 Dec 11. 394(4):732-46.
  PUBMED    19782090
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Dec 11. 394(4):732-46."
COMMENT     Sequence Label: UbG76V-eGFP-V5His_15aa
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    254..375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(291..310)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     345..364
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(519..540)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagtcatgt cggccgctcg agtctagagg gcccttcgaa
       61 ggtaagcctt gacctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac
      121 catcaccatt gagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
      181 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      241 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      301 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      361 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
      421 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
      481 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
      541 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
      601 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
      661 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
      721 cgagaaagga agggagaaag cgaaaggagc
//
