LOCUS       sequence-298112-        1920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 nsp6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1920)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046499
FEATURES             Location/Qualifiers
     source          1..1920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             675..692
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(1835..1853)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ttttgtgctt attcttgttg ccttctctcg ctacggtagc ttatttcaat atggtctaca
       61 tgccagcttc ctgggttatg cgtatcatga cttggttgga catggttgat accagccttt
      121 ctggtttcaa gttgaaagat tgtgtcatgt acgcatctgc cgttgtatta ctaattctta
      181 tgactgctag aaccgtctat gacgatggcg cgagaagggt ttggacacta atgaacgtgt
      241 tgactttagt ttacaaggtc tactatggaa atgccttgga ccaagctatc tccatgtggg
      301 ctttaataat ctcagtcact tctaactaca gtggtgttgt tactacagtg atgtttttgg
      361 ctagaggtat tgtcttcatg tgtgttgaat attgtcccat tttctttatc accggtaaca
      421 ctttacaatg cattatgttg gtttactgtt tcttaggtta cttttgtacc tgttatttcg
      481 gcctattttg tttgttgaat cgatacttta gactcacatt gggtgtttac gattatttgg
      541 tcagcactca agaatttaga tatatgaact cccaaggttt gttaccacca aagaactcta
      601 ttgatgcctt caagttgaac atcaagctgt taggtgtcgg tggaaaacct tgcatcaagg
      661 tagccaccgt ccaactcgtg ccgagaggtt ctaaaatcga ggaaggtaag ctagtcattt
      721 ggataaatgg agataagggt tacaacggtt tggcagaagt aggcaagaaa tttgaaaagg
      781 acacaggtat caaggtgacg gttgaacatc cagacaagtt agaagaaaaa ttccctcaag
      841 ttgctgctac tggtgatggt cccgacatta ttttctgggc ccacgatcgt tttggtggat
      901 atgctcagtc aggtttgctg gctgagatca ccccagacaa ggccttccaa gataaattgt
      961 acccattcac ttgggatgct gtcagataca acggtaagct tatagcatat ccaattgctg
     1021 ttgaagcctt atcgttgatc tacaataagg acttgttacc taacccacca aagacatggg
     1081 aagaaattcc agctttggat aaagagctaa aggccaaggg aaaaagcgct ttgatgttta
     1141 acttacaaga accttatttc acctggccat taatcgctgc tgacggtggt tacgcattta
     1201 aatacgaaaa cggtaagtat gacattaagg atgtcggcgt tgataatgct ggtgcaaagg
     1261 ctggtttgac cttcttggtt gatttaataa agaataaaca catgaacgct gacactgact
     1321 actctatcgc cgaagccgct ttcaacaagg gtgaaactgc tatgaccata aatggtccat
     1381 gggcctggag taacattgat acttctaaag taaactatgg tgtcactgtt ttgcctacat
     1441 tcaaaggcca accatccaag ccctttgtcg gtgttctatc cgctggcatc aatgctgctt
     1501 ctccaaacaa ggagctagct aaagaatttt tggaaaatta cttgttaact gacgaaggtt
     1561 tggaggccgt taacaaggac aagccattgg gtgctgtcgc tttgaaatct tacgaagaag
     1621 aattggctaa ggatcccaga attgccgcta ccatggagaa cgcccaaaag ggtgaaatta
     1681 tgccaaacat cccacaaatg tcagccttct ggtacgcagt taggactgcc gtgattaatg
     1741 ctgcttccgg tagacaaacc gtcgacgaag cattaaaaga tgctcaaacg taaacaggcc
     1801 ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca cgccctcctc
     1861 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
//
