LOCUS       sequence-298266-        9968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone for CRISPR-Cas9(H840A) prime editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Tang X, Sretenovic S, Ren Q, Jia X, Li M, Fan T, Yin D, Xiang S, Guo
            Y, Liu L, Zheng X, Qi Y, Zhang Y
  TITLE     Plant Prime Editors Enable Precise Gene Editing in Rice Cells.
  JOURNAL   Mol Plant. 2020 Mar 25. pii: S1674-2052(20)30072-1. doi: 
            10.1016/j.molp.2020.03.010.
  PUBMED    32222487
REFERENCE   2  (bases 1 to 9968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2020 Mar 25. pii: S1674-2052(20)30072-1. doi: 
            10.1016/j.molp.2020.03.010."
COMMENT     Sequence Label: pYPQ166-OsPE2
FEATURES             Location/Qualifiers
     source          1..9968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2582..2647
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2654..2674
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2699..5212,5213..5215,5216..6799)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6712..6720
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6800..6847
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9020..9040
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    9715..9838
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9855..9874)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9856..9874)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9879..9895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9879..9895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 ggattacaag gaccacgacg gggattacaa ggaccacgac attgattaca aggatgatga
     2641 tgacaagatg gctccgaaga agaagaggaa ggttggcatc cacggggtgc cagctgctga
     2701 caagaagtac tcgatcggcc tcgatattgg gactaactct gttggctggg ccgtgatcac
     2761 cgacgagtac aaggtgccct caaagaagtt caaggtcctg ggcaacaccg atcggcattc
     2821 catcaagaag aatctcattg gcgctctcct gttcgacagc ggcgagacgg ctgaggctac
     2881 gcggctcaag cgcaccgccc gcaggcggta cacgcgcagg aagaatcgca tctgctacct
     2941 gcaggagatt ttctccaacg agatggcgaa ggttgacgat tctttcttcc acaggctgga
     3001 ggagtcattc ctcgtggagg aggataagaa gcacgagcgg catccaatct tcggcaacat
     3061 tgtcgacgag gttgcctacc acgagaagta ccctacgatc taccatctgc ggaagaagct
     3121 cgtggactcc acagataagg cggacctccg cctgatctac ctcgctctgg cccacatgat
     3181 taagttcagg ggccatttcc tgatcgaggg ggatctcaac ccggacaata gcgatgttga
     3241 caagctgttc atccagctcg tgcagacgta caaccagctc ttcgaggaga accccattaa
     3301 tgcgtcaggc gtcgacgcga aggctatcct gtccgctagg ctctcgaagt ctcggcgcct
     3361 cgagaacctg atcgcccagc tgccgggcga gaagaagaac ggcctgttcg ggaatctcat
     3421 tgcgctcagc ctggggctca cgcccaactt caagtcgaat ttcgatctcg ctgaggacgc
     3481 caagctgcag ctctccaagg acacatacga cgatgacctg gataacctcc tggcccagat
     3541 cggcgatcag tacgcggacc tgttcctcgc tgccaagaat ctgtcggacg ccatcctcct
     3601 gtctgatatt ctcagggtga acaccgagat tacgaaggct ccgctctcag cctccatgat
     3661 caagcgctac gacgagcacc atcaggatct gaccctcctg aaggcgctgg tcaggcagca
     3721 gctccccgag aagtacaagg agatcttctt cgatcagtcg aagaacggct acgctgggta
     3781 cattgacggc ggggcctctc aggaggagtt ctacaagttc atcaagccga ttctggagaa
     3841 gatggacggc acggaggagc tgctggtgaa gctcaatcgc gaggacctcc tgaggaagca
     3901 gcggacattc gataacggca gcatcccaca ccagattcat ctcggggagc tgcacgctat
     3961 cctgaggagg caggaggact tctacccttt cctcaaggat aaccgcgaga agatcgagaa
     4021 gattctgact ttcaggatcc cgtactacgt cggcccactc gctaggggca actcccgctt
     4081 cgcttggatg acccgcaagt cagaggagac gatcacgccg tggaacttcg aggaggtggt
     4141 cgacaagggc gctagcgctc agtcgttcat cgagaggatg acgaatttcg acaagaacct
     4201 gccaaatgag aaggtgctcc ctaagcactc gctcctgtac gagtacttca cagtctacaa
     4261 cgagctgact aaggtgaagt atgtgaccga gggcatgagg aagccggctt tcctgtctgg
     4321 ggagcagaag aaggccatcg tggacctcct gttcaagacc aaccggaagg tcacggttaa
     4381 gcagctcaag gaggactact tcaagaagat tgagtgcttc gattcggtcg agatctctgg
     4441 cgttgaggac cgcttcaacg cctccctggg gacctaccac gatctcctga agatcattaa
     4501 ggataaggac ttcctggaca acgaggagaa tgaggatatc ctcgaggaca ttgtgctgac
     4561 actcactctg ttcgaggacc gggagatgat cgaggagcgc ctgaagactt acgcccatct
     4621 cttcgatgac aaggtcatga agcagctcaa gaggaggagg tacaccggct gggggaggct
     4681 gagcaggaag ctcatcaacg gcattcggga caagcagtcc gggaagacga tcctcgactt
     4741 cctgaagagc gatggcttcg cgaaccgcaa tttcatgcag ctgattcacg atgacagcct
     4801 cacattcaag gaggatatcc agaaggctca ggtgagcggc cagggggact cgctgcacga
     4861 gcatatcgcg aacctcgctg gctcgccagc tatcaagaag gggattctgc agaccgtgaa
     4921 ggttgtggac gagctggtga aggtcatggg caggcacaag cctgagaaca tcgtcattga
     4981 gatggcccgg gagaatcaga ccacgcagaa gggccagaag aactcacgcg agaggatgaa
     5041 gaggatcgag gagggcatta aggagctggg gtcccagatc ctcaaggagc acccggtgga
     5101 gaacacgcag ctgcagaatg agaagctcta cctgtactac ctccagaatg gccgcgatat
     5161 gtatgtggac caggagctgg atattaacag gctcagcgat tacgacgtcg atgctatcgt
     5221 tccacagtca ttcctgaagg atgactccat tgacaacaag gtcctcacca ggtcggacaa
     5281 gaaccggggc aagtctgata atgttccttc agaggaggtc gttaagaaga tgaagaacta
     5341 ctggcgccag ctcctgaatg ccaagctgat cacgcagcgg aagttcgata acctcacaaa
     5401 ggctgagagg ggcgggctct ctgagctgga caaggcgggc ttcatcaaga ggcagctggt
     5461 cgagacacgg cagatcacta agcacgttgc gcagattctc gactcacgga tgaacactaa
     5521 gtacgatgag aatgacaagc tgatccgcga ggtgaaggtc atcaccctga agtcaaagct
     5581 cgtctccgac ttcaggaagg atttccagtt ctacaaggtt cgggagatca acaattacca
     5641 ccatgcccat gacgcgtacc tgaacgcggt ggtcggcaca gctctgatca agaagtaccc
     5701 aaagctcgag agcgagttcg tgtacgggga ctacaaggtt tacgatgtga ggaagatgat
     5761 cgccaagtcg gagcaggaga ttggcaaggc taccgccaag tacttcttct actctaacat
     5821 tatgaatttc ttcaagacag agatcactct ggccaatggc gagatccgga agcgccccct
     5881 catcgagacg aacggcgaga cgggggagat cgtgtgggac aagggcaggg atttcgcgac
     5941 cgtcaggaag gttctctcca tgccacaagt gaatatcgtc aagaagacag aggtccagac
     6001 tggcgggttc tctaaggagt caattctgcc taagcggaac agcgacaagc tcatcgcccg
     6061 caagaaggac tgggatccga agaagtacgg cgggttcgac agccccactg tggcctactc
     6121 ggtcctggtt gtggcgaagg ttgagaaggg caagtccaag aagctcaaga gcgtgaagga
     6181 gctgctgggg atcacgatta tggagcgctc cagcttcgag aagaacccga tcgatttcct
     6241 ggaggcgaag ggctacaagg aggtgaagaa ggacctgatc attaagctcc ccaagtactc
     6301 actcttcgag ctggagaacg gcaggaagcg gatgctggct tccgctggcg agctgcagaa
     6361 ggggaacgag ctggctctgc cgtccaagta tgtgaacttc ctctacctgg cctcccacta
     6421 cgagaagctc aagggcagcc ccgaggacaa cgagcagaag cagctgttcg tcgagcagca
     6481 caagcattac ctcgacgaga tcattgagca gatttccgag ttctccaagc gcgtgatcct
     6541 ggccgacgcg aatctggata aggtcctctc cgcgtacaac aagcaccgcg acaagccaat
     6601 cagggagcag gctgagaata tcattcatct cttcaccctg acgaacctcg gcgcccctgc
     6661 tgctttcaag tacttcgaca caactatcga tcgcaagagg tacacaagca ctaaggaggt
     6721 cctggacgcg accctcatcc accagtcgat taccggcctc tacgagacgc gcatcgacct
     6781 gtctcagctc gggggcgaca agcggccagc ggcgacgaag aaggcggggc aggcgaagaa
     6841 gaagaagtca gggggttcta gcggcggttc atctggctcc gaaacaccgg gaacttccga
     6901 gagtgctact ccagaatcta gcggtgggtc atctggaggg tcttccacac ttaatattga
     6961 ggacgaatac cgcctgcatg aaactagtaa agaacccgac gtgagcctcg gtagcacgtg
     7021 gctctctgat ttcccgcaag catgggctga aacaggtggg atgggcctcg ctgttcgcca
     7081 ggctccattg atcatcccac tcaaggccac ttctacaccg gtgtccataa agcaataccc
     7141 aatgagccag gaagctcggc ttggtatcaa gcctcacatc caaagattgc ttgatcaggg
     7201 aattcttgtc ccttgtcaaa gtccatggaa cacccctctt cttcccgtca aaaaacctgg
     7261 gactaacgat tataggccag tgcaagacct cagggaggtt aacaaaaggg ttgaagacat
     7321 tcatccaaca gtgccgaatc cttataatct gctctcaggt ttgcctccaa gtcaccagtg
     7381 gtatacagtg ctggatctta aagacgcatt tttttgtctg cgccttcatc ccacttcgca
     7441 acctcttttt gctttcgaat ggagagatcc agagatgggt atctccggac agctcacatg
     7501 gacccggttg ccccaaggct tcaaaaactc cccaaccttg tttaatgaag cgctgcacag
     7561 agatttggcg gacttcagga tccagcaccc cgacttgatt ctcctgcagt atgtggatga
     7621 ccttttgctt gcagccacat ctgagcttga ctgccaacag gggacgcgcg cccttcttca
     7681 gactttggga aaccttgggt atagagcctc tgccaagaag gcacagatct gccagaagca
     7741 agttaagtat ctcggttatt tgctcaagga gggtcagagg tggctgacgg aggcgaggaa
     7801 agaaacggtg atgggccaac caaccccaaa aacccctagg caacttagag agtttcttgg
     7861 taaagcgggg ttttgccggt tgttcattcc aggctttgcc gagatggccg caccgttgta
     7921 tccattgacg aaaccgggca ccctttttaa ttgggggccc gaccagcaga aggcgtatca
     7981 ggagatcaag caagctctcc tgaccgcacc agcactcgga cttccagatc ttacgaaacc
     8041 gtttgagctc ttcgtggatg agaaacaggg gtatgctaag ggagtcctca cacaaaagtt
     8101 gggaccgtgg aggcggccgg ttgcctatct ttcgaaaaaa cttgatcccg tggcggctgg
     8161 ttggcctccc tgtctgagga tggtggccgc gatcgcagtc cttacaaaag atgcagggaa
     8221 gttgactatg ggacaaccat tggtcatact tgccccgcac gcggttgagg ccctcgttaa
     8281 gcagcctccc gataggtggc tcagtaacgc ccgcatgact cattaccagg cactgcttct
     8341 cgacacagac agggtgcagt ttggtcccgt cgtcgctttg aaccccgcca ccctgctgcc
     8401 cctcccggag gaaggattgc agcacaactg tctggatatc ttggccgaag cgcatgggac
     8461 tcgcccggac ttgactgacc agccccttcc cgacgctgac catacttggt atacggatgg
     8521 aagcagcctt ctgcaggaag ggcaacgcaa ggcaggggcc gcggttacta ctgaaactga
     8581 ggtcatctgg gctaaggctt tgccagccgg aactagtgct caaagagcag aattgattgc
     8641 cctcacgcaa gctttgaaaa tggctgaggg taagaagctt aatgtgtata cagacagtag
     8701 gtatgcattt gcgacagcac atatccatgg tgagatctac cggcgcagag gatggcttac
     8761 gagcgagggg aaggagatta agaacaaaga tgagattttg gccctgctca aagcgctctt
     8821 cctcccgaag cgccttagca tcatccattg cccaggccat cagaagggtc actcggccga
     8881 agcacggggt aataggatgg ccgatcaggc agcaaggaag gctgctatca ctgagacacc
     8941 cgacacctct acccttctca tagagaattc atcgccgagc ggcggcagca agaggactgc
     9001 agacgggtcg gaattcgaac cgaagaaaaa gagaaaggtg tgagctcaga gctttcgttc
     9061 gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac
     9121 cagaatccta ctgagtttga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     9181 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     9241 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     9301 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     9361 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     9421 agtaaaacac ttgtagttgt accattatgc ttattcacta ggcaacaaat atattttcag
     9481 acctagaaaa gctgcaaatg ttactgaata caagtatgtc ctcttgtgtt ttagacattt
     9541 atgaactttc ctttatgtaa ttttccagaa tccttgtcag attctaatca ttgctttata
     9601 attatagtta tactcatgga tttgtagttg agtatgaaaa tattttttaa tgcattttat
     9661 gacttgccaa ttgttccgga actctagata agcttaccgg aaagggcgaa ttcgcaactt
     9721 tgtatacaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     9781 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatgcc
     9841 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     9901 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     9961 gcctcctc
//
