LOCUS       sequence-298295-        4734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4734)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046673
FEATURES             Location/Qualifiers
     source          1..4734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(489..507)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             858..1661
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      1744..2726
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      2804..2833
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2855..3442
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3454..3481)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3505..3696)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(3697..4488,4489..4557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4320..4339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4558..4660)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(4661..4692)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat
       61 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
      121 ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
      181 aaaagagacc gcctcgtttc tttttcttcg cgtttctttt tcttgaaaat tttttttttt
      241 gatttttttc tctttcgatg acctcccatt gatatttaag ttaataaacg gtcttcaatt
      301 tctcaagttt cagtttcatt tttcttgttc tattacaact ttttttactt cttgctcatt
      361 agaaagaaag catagcaatc taatctaagt tttaattaca aaatgtccgc tgacgcacaa
      421 tcattcctaa atgggtttgc tgtataaaca ggcccctttt cctttgtcga tatcatgtaa
      481 ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac cgaaaaggaa
      541 ggagttagac aacctgaagt ctaggtccct atttattttt tttaatagtt atgttagtat
      601 taagaacgtt atttatattt caaatttttc ttttttttct gtacaaagcg gccgccgaac
      661 cactagtgca ctgcagtaca cggtttcctt gaaatttttt tgattcggta atctccgaac
      721 agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg
      781 ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacgtgca
      841 ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta
      901 gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt
      961 cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa
     1021 tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta
     1081 agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaggac agaaaatttg
     1141 ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat
     1201 gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc
     1261 aggcggcaga agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat
     1321 gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg
     1381 acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt
     1441 acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagat gcattgggtc
     1501 aacagtatag aaccgtggat gatgtggttt ctacaggatc tgacattatt attgttggaa
     1561 gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag
     1621 gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa
     1681 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattacccg
     1741 agtaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
     1801 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
     1861 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
     1921 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     1981 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     2041 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     2101 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     2161 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     2221 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     2281 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     2341 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
     2401 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
     2461 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
     2521 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
     2581 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
     2641 gtccggtgcg tttttggttt tttgaaagtg cgtcatcaga gcgcttttgg ttttcaaaag
     2701 cgctctgaag ttcctatact ttctagctag agaataggaa cttcccgagc ggccgcgtgt
     2761 tacaagcttg actccagcgt aactggactg caatcaactc actggctcac cttcacgggt
     2821 gggcctttct tcggtagaaa atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2881 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2941 agctaccaac tctttttccg aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3001 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3241 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca ggggtaaacg cctggtatct
     3361 ttatagtcct gtcgggtttc gccacctctg acttgagcat cgatttttgt gatgctcgtc
     3421 aggggggcgg agcctatgga aaaacgccag caacgcagaa aggcccaccc gaaggtgagc
     3481 caggtgatta catttgggcc ctcatcagag gttttcaccg tcatcaccga aacgcgcgag
     3541 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
     3601 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     3661 ggccatgtta agggcggttt tttcctgttt ggtcacttac caatgcttaa tcagtgaggc
     3721 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3781 gataactacg atgcgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3841 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggga gggccgagcg
     3901 cagaagtgat cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3961 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4021 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4081 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4141 cgttgccaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4201 ttctcttact gtcatgccat ccgtgagatg cttttctgtg actggtgagt actcaaccaa
     4261 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4321 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4381 gcgtaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4441 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4501 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatttt
     4561 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     4621 ttcattatgg tgaaagttgg aacctcttac gtgccgatca agtcaaaagc ctccggtcgg
     4681 aggcttttga ctttctgcta tggaggtcag gtatgattta aatggtcagt taga
//
