LOCUS       sequence-298305-         489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 
            promoter. The cloning site design allows a one-step cloning strategy
            of both sgRNAs. Expresses a Thy1.1 marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, 
            Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber 
            J, Obenauf AC
  TITLE     Isolating live cell clones from barcoded populations using 
            CRISPRa-inducible reporters.
  JOURNAL   Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0.
  PUBMED    32719478
REFERENCE   2  (bases 1 to 489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0."
COMMENT     Sequence Label: Dual-sgRNA_hU6-mU6
FEATURES             Location/Qualifiers
     source          1..489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..489
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
ORIGIN
        1 atgaacccag ccatcagcgt cgctctcctg ctctcagtct tgcaggtgtc ccgagggcag
       61 aaggtgacca gcctgacagc ctgcctggtg aaccaaaacc ttcgcctgga ctgccgccat
      121 gagaataaca ccaaggataa ctccatccag catgagttca gcctgacccg agagaagagg
      181 aagcacgtgc tctcaggcac cctcgggata cccgagcaca cgtaccgctc ccgcgtcacc
      241 ctctccaacc agccctatat caaggtcctt accctagcca acttcaccac caaggatgag
      301 ggcgactact tttgtgagct tcgagtctcg ggcgcgaatc ccatgagctc caacaaaagt
      361 atcagtgtgt atagagacaa actggtcaag tgtggcggca taagcctgct ggttcagaac
      421 acatcctgga tgctgctgct gctgctttcc ctctccctcc tccaagccct ggacttcatt
      481 tctctgtga
//
