LOCUS       sequence-298526-        1821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of biosynthetic enzyme 4CL in fusion with CC segment P3S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala
            R, Gradisar H
  TITLE     Metabolic enzyme clustering by coiled coils improves the 
            biosynthesis of resveratrol and mevalonate.
  JOURNAL   AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
  PUBMED    32448937
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2020-05-24"; volume: "10(1):97. doi"; pages: " 
            10.1186/s13568-020-01031-5"
COMMENT     Sequence Label: pET19b_4CL:P3S
FEATURES             Location/Qualifiers
     source          1..1821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1756..1770)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(1786..1815)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttactgctgt tcgaactgga actggaattt ctgctgggag ccgccggagc cggagccgga
       61 gcccccaccc agaccatttg ccagttttgc acgcagatct ttacgcagaa ttttaccgct
      121 cggtgctttc ggaatgctct cggtaaaaaa cactttgttg atgcgtttgt agaacaccac
      181 ctgtttgcta acaaactgtt taacatcatc ttcgctcagt tcgctatctt tgcttttcac
      241 aacaaatgca accggaactt cgcctgctgc ttcttctttc attgcaacaa ctgcaacatc
      301 ggtaatatcc ggatgaccaa tcagcagtgc ttccagttct gccggtgcaa cctgaaaacc
      361 tttgtatttg atcagctctt tcagacgatc cacaataaac agttcgtcat catcatcaat
      421 cagaccaata tcacctgtat gcagccaacc atctttatca atggtttctg cggttgctgc
      481 cggattattc agatagcctt tcataatctg gtgaccacga atacaaattt caccaggctg
      541 attacggctc aggctatcac cggtatccgg atcaacaatt ttcatttctg cattacgaac
      601 aacggtgcca catgcaccac ttttaaccgg aaacggttct tttgcaaaac ccaggctcat
      661 tgccagaacc ggacctgctt cggtcatacc ataaccctga cccagttttg cattcggaaa
      721 tttggcatta actgcatctt ccagttcttt acccagcgga gctgcaccgc ttttaacaac
      781 acgaatgctg ctcagatcat atttttcggt ttcgctgctt tttgcaattg ccagaacaat
      841 aggcggaacc atcggtgcaa cggtaacttt acaacgctgg atcagttcca gcagcagatt
      901 aatttcaaat ttcggcataa tcagaattgc tgcaccaaca cgcagaccac acagcataat
      961 gctattcagt gcataaatat gaaacatcgg cagcacacac agaataacat cgtcgctatg
     1021 aaaatacaga ttcggatttt caccatcaac ctgctgtgca acgctggtaa ccagaccttt
     1081 atgggtcagc ataacacctt tcggcagacc ggtggtgccg ctgctatacg gcagtgcaac
     1141 aacatcatcc ggactaattt caacgctatc aataacttcg cttgcttcgg tggtgctctg
     1201 ggtcagttcg gtaaaacgca gacaaccttc cggaatcgga acgctttcat tatcatcgat
     1261 gcaaacaatc acaacaccat catcattttg cagcggtttg attttgtcca cgtaacgtgc
     1321 ttcggtaata atcagtttgg tgttgcttgc ttttgcctgt tttgcaattt ctgccggtgt
     1381 aaaaaacgga tttgctgcgg ttgcggttgc accacgaaag ctggctgcca gaaagctcag
     1441 aacaaattcc ggacaattcg gcagcagcag cataacaacg tcattctgat tcacacccag
     1501 tttatgaaaa ttggctgcaa tctgacggct gatcacatga acatcactat aggtataaac
     1561 atgaccggtc ggaccattaa tcagacacgg tttggtggca aactcgctaa tgttctgaaa
     1621 gatataatcg tgcaggctca gatgattcgg aatatagata tccggcagtt tgctacgaaa
     1681 aatcacgtcg ctgttgttat tgttgctctg tttttccata acctggctaa ctgcctgttc
     1741 ttgcggtgcc atatgcttgt cgtcgtcgtc gatatggccg ctgctgtgat gatgatgatg
     1801 atgatgatga tgatggccca t
//
