LOCUS       sequence-298735-        9976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An all-in-one bicistronic plasmid for controlling protein 
            degradation by AID technology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Yesbolatova A, Saito Y, Kanemaki MT
  TITLE     Constructing Auxin-Inducible Degron Mutants Using an All-in-One 
            Vector.
  JOURNAL   Pharmaceuticals (Basel). 2020 May 23;13(5). pii: ph13050103. doi: 
            10.3390/ph13050103.
  PUBMED    32456235
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pharmaceuticals (Basel)."; date: "2020-05-23"; volume: "13(5). pii:
            ph13050103. doi"; pages: " 10.3390/ph13050103"
COMMENT     Sequence Label: pAID5.2-C
FEATURES             Location/Qualifiers
     source          1..9976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(679..690,691..853)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(884..917)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1474..2073)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2054..2073
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2113..2612)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2161..2180)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2554..2577
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(2673..2706)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2733..2854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2744..2763)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2798..2817
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(2972..3685,3686..3688,3689..3691))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2975..3685,3686..3688,3689..3691))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3017..3038)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3366..3385
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3625..3646
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3698..3901)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     misc_feature    3914..3970
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(3971..4027)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4043..5767)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(5812..5831)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5839..6855
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5893..5915)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6856..7133)
                     /label=chicken beta-actin promoter
     enhancer        7135..7514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(7536..7723,7724..7735)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7792..7812)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7792..7810)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7831..7847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7831..7847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7844..7866)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7855..7871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7879..7885,7886..7903,7904..7909))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7924..7945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8062..8079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8233..8821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8313..8332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8992..9783,9784..9852))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9615..9634
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9853..9957)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc nggcgggtgt ggtggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
      721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
      841 gtaaaagcaa gaaagatctt agtgaacctc ttcgagggac ctaataactt cgtatagcat
      901 acattatacg aagttatatt aagggttccg gatctcgacc agcttctgat ggaattagaa
      961 cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg atctcgttct
     1021 tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa agtgggaatc
     1081 aagagaaaaa caatgatccc acgagagatc tatagatcta tagatcatga gtgggaggaa
     1141 tgagctggcc cttaatttgg ttttgcttgt ttaaattatg atatccaact atgaaacatt
     1201 atcataaagc aatagtaaag agccttcagt aaagagcagg catttatcta atcccacccc
     1261 acccccaccc ccgtagctcc aatccttcca ttcaaaatgt aggtactctg ttctcaccct
     1321 tcttaacaaa gtatgacagg aaaaacttcc attttagtgg acatctttat tgtttaatag
     1381 atcatcaatt tctgcatccc ggggatctga tatcatcgat gcatggggtc gtgcgctcct
     1441 ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc gggtcatgca
     1501 ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc
     1561 gtagaagggg aggttgtggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc
     1621 ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg
     1681 cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag
     1741 gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc
     1801 gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc
     1861 ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag
     1921 ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc
     1981 ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt
     2041 ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttcagctgct cgagatctag
     2101 atggatgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg
     2161 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
     2221 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
     2281 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
     2341 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
     2401 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
     2461 gaaggggcca ccaaagaacg gagtcggttg gcgcctaccg gtggatgtgg aatgtgtgcg
     2521 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
     2581 agactgcctt gggaaaagcg cctcccctac ccggtagaat ttcgaggtcg agatcctaag
     2641 cttggctgga cgtaaactcc tcttcagacc taataacttc gtatagcata cattatacga
     2701 agttatatta agggttattg aatatgatcg cttaagatac attgatgagt ttggacaaac
     2761 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2821 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2881 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     2941 tggtatggct gattatgatc agttatctag attacttgta cagctcgtcc atgccgagag
     3001 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     3061 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     3121 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     3181 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     3241 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     3301 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     3361 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     3421 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     3481 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     3541 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     3601 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     3661 cggtgaacag ctcctcgccc ttgctcacca tgctagcttt atacatcctc aaatcgattt
     3721 tcctcaagta cggtgctccg tccattgata ccttcacgaa cgccgccgcc tccgggccac
     3781 cgcttgattt ttggcaggaa accatcacgt tcttccggta tgatctcacc ggtggccatc
     3841 ccacaacttg tgccttggcc ggaggtttgg ctggatcttt aggacaagca ctcttctcct
     3901 tggcgcctgc accggatccc gggcccgcgg taccgtcgac tgcagaattc gaagcttgag
     3961 ctcgagatct aggtccaggg ttctcctcca cgtctccagc ctgcttcagc aggctgaagt
     4021 tagtagctcc gcttcctgat ctcagaatct tcacaaagtt gggagcatca tctcttgccc
     4081 ctgcggtggt ccgatacaca tacagtttct ccacctttgg caggtcgcca tgattttcct
     4141 ccatttcgtt agacccgtcc cgctcattaa tgacttccac gttcagcata ggcattttac
     4201 tagccaggac ctgacagccc ttcagagtca cattgcaact agacatccac aggctcctca
     4261 ttgtctcgta tctggcaaag tttcccagca gagcggcatc cccgaaaggg ctgtcccgga
     4321 tttccagttt tcgcagattc ttacacccgt tcatcacgtg catcatgccc ttatcggagt
     4381 ctccggcaaa tgcaatgctc agcatctcca gctgcttagc atacttgccg atgtacatga
     4441 acactttgtc tgtcagcagg ccggaaatgc tcagcctctg gagtcccttg cactctctca
     4501 cgatagctcc aaacccttca tccagtggct gggatgtgac cacgtcgggt ttgcctggtt
     4561 ccagaataca cagccgaaat cgggtgaaat tagggcagtt cttggcgacg gtcaccagtg
     4621 cagcattagt catctgatga cagaaataca gcaggctgtt cagctttggg catcccagtg
     4681 agactgccac caggccttcc tcagtgacag cactatatcc ggccacgtaa aaatcagatg
     4741 ggaacactct cagctcctgg aggtccttgc aggagctggc gaccacctgg agccccttat
     4801 cggaaataca gtccagcacc cacagtcgct ggagtttcac gcagcgtgag atcatctttg
     4861 tcagatcact ggcgtccagg gttggtgcat agctcaggtt caggcctgtc agctgtgcac
     4921 acagagggta gataaagctc aggcacacgg gtgaagcatc ccagaatcct gacagactcc
     4981 tcagcatttt acacttctcc agggcagaag tcagtttaaa gtaggattct gtctggaaat
     5041 cgtctgtcag attgccggtt cccagatcct ccaggttagg ggttctcagc agaatcttag
     5101 ccagagtgtc cacgctcacg ctccggttca gccgcagaga cctcaggttg gggcttcttg
     5161 agaccagccg ctccagggac ccagcattca cttcgccttt gatgcaggca aagttcaggg
     5221 acaccaggct ggtacatgag tctgggaagc aagacagcca tctggggcct ctatcctcca
     5281 cttcattctc ctggaggtcc agctcccgca gcagcttaca gtgggaggcc acggctgcca
     5341 gtccatcggt gctgaagcct tcgcaagaaa tcagcaccag tgcgcgaaac ctagggaagc
     5401 tccgagccag cagctccaga ctttcgtcag agaccaccat tcgcttcatg cgcagttcct
     5461 ccagtccatg acagccgcga gcggctgcct cgatccaagg gccggcgtat cctccccaat
     5521 cagggggcac caggttaaag tcggcgaagt ggggtttccc cttgacggtc agtgcgcgca
     5581 catttggaaa cctagcggcg accctgcctg ctctcacagc atagcagttg ccgacgaaca
     5641 ctcctctccg gctcaggcgt tcgatctcgt accacacttt gcagaccagg ctcactgtgt
     5701 ttctatctct ctgtgcaggc aggaagctaa aaatgtgttc gacgacctct tcaggaaagt
     5761 atgtcatggt ggcagcgctt cgaggaattc tttgccaaaa tgatgagaca gcacaataac
     5821 cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc
     5881 tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag
     5941 gcagccgtcc ccccgcggac agccccgagg ctggagaggg agaaggggac ggcggcgcgg
     6001 cgacgcacga aggccctccc cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc
     6061 gcgcccgcta gagggggtgc ggcggcgcct cccagatttc ggctccgcac agatttggga
     6121 caaaggaagt ccctgcgccc tctcgcacga ttaccataaa aggcaatggc tgcggctcgc
     6181 cgcgcctcga cagccgccgg cgctccgggg gccgccgcgc ccctcccccg agcccycccc
     6241 ggcccgaggc ggccccgccc cgcccggcac ccccacctgc cgccaccccc cgcccggcac
     6301 ggcgagcccc gcgccacgcc ccgtacggag ccccgcaccc gaagccgggc cgtgctcagc
     6361 aactcgggga ggggggtgca gggggggttg cagcccgacc gacgcgccca caccccctgc
     6421 tcaccccccc acgcacacac cccgcacgca gcctttgttc ccctcgcagc cccccccgca
     6481 ccgcggggca ccgcccccgg ccgcgctccc ctcgcgcaca ctgcggagcg cacaaagccc
     6541 cgcgccgcgc ccgcagcgct cacagccgcc gggcagcgcg gagccgcacg cggcgctccc
     6601 cacgcacaca cacacgcacg caccccccga gccgctcccc ccgcacaaag ggccctcccg
     6661 gagcccctca aggctttcac gcagccacag aaaagaaaca agccgtcatt aaaccaagcg
     6721 ctaattacag cccggaggag aagggccgtc ccgcccgctc acctgtggga gtaacgcggt
     6781 cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag cggggcacgg ggcgaaggca
     6841 gcgcgcagcg actcccgccc gccgcgcgct tcgcttttta tagggccgcc gccgccgccg
     6901 cctcgccata aaaggaaact ttcggagcgc gccgctctga ttggctgccg ccgcacctct
     6961 ccgcctcscc ccsccccscc ccycsccccs ccccsccccs ccyggcgcgc gccccccccc
     7021 cccccccgcc cccatcgctg cacaaaataa ttaaaaaata aataaataca aaattggggg
     7081 tggggagggg ggggagatgg ggagagtgaa gcagaacgtg gggctcacct cgaccatggt
     7141 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
     7201 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
     7261 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
     7321 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
     7381 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
     7441 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
     7501 caataatcaa tgtcgagcat gcatctagag ggcccaagtg atctccaaaa aataagtact
     7561 ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa
     7621 ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa
     7681 aataatactt aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct
     7741 tgacccccta ccttcagcaa gcccagcaga tccgagctcc agcttttgtt ccctttagtg
     7801 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7861 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7921 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7981 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8041 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8641 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9061 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9121 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9181 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9241 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9301 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9361 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9421 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9481 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9541 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9601 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9661 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9721 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9781 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9841 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9901 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9961 atttccccga aaagtg
//
