LOCUS       sequence-299052-        2178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0384
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     97..115
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(119..162)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      316..373
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(397..414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(568..1156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(648..667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1339..1433)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1454..2113)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2028..2047
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
       61 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
      121 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcagga
      181 gggaggcgtg tcgttttagt gaggtgaagc atcttcctga agcatcttcc tgaagcatct
      241 tccctatata aggttttgct attcattgaa agcagtagtg actgatttgt atataatgta
      301 ctcgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
      361 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
      421 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      481 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      541 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
      601 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      661 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      721 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      781 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      841 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      901 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      961 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1021 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1081 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1141 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1201 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1261 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1321 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1381 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     1441 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1501 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1561 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1621 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1681 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1741 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1801 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1861 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1921 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1981 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     2041 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     2101 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     2161 ccggtagtga tcttattt
//
