LOCUS       sequence-299288-        2487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0084
FEATURES             Location/Qualifiers
     source          1..2487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     460..479
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     707..725
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(729..772)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     1157..1177
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        1166..1212
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1235..1292
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1316..1333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1487..2075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1567..1586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2258..2352)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2373..2487,1..545))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
       61 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
      121 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
      181 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
      241 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
      301 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
      361 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
      421 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
      481 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
      541 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
      601 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
      661 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      721 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
      781 ggtctcagga ggtcaacatg gtggagcacg acactctggt ctactccaaa aatgtcaaag
      841 atacagtctc agaagatcaa agggctattg agacttttca acaaaggata atttcgggaa
      901 acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca gtagaaaagg
      961 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt caagaagcaa
     1021 gtggatgatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt tcctctctct
     1081 gccgacagtg gtcccaaact gacgtaaggg atgacgcaca tgatgtgaca tctccaaacc
     1141 acgtctacaa atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
     1201 atttggagag gacacgctac tcgagaccct gcagtccggc aaaaaagggc aaggtgtcac
     1261 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc
     1321 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1381 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1441 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
     1501 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1561 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1621 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1681 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1741 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1801 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1861 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1921 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1981 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2041 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2101 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2161 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2221 tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc
     2281 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     2341 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     2401 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     2461 acctgaatcg ccagcggcat cagcacc
//
