LOCUS       sequence-299291-        2519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0115
FEATURES             Location/Qualifiers
     source          1..2519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     117..136
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     364..382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(386..429)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      924..981
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1005..1022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1176..1764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1256..1275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1947..2041)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2062..2519,1..202))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
       61 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
      121 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
      181 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
      241 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
      301 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
      361 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      421 gatgatttct ggaattcggt ctcaggagtg gaagattcag aaaatctcca tcaacaaata
      481 atccaagtaa ggattaatgg attgatcaac atccttaccg ctatgggtaa gattgatgaa
      541 aagtcaaaaa caaaaatcaa ttatgcacac cagcatgtgt tgatcaccag ctattgtggg
      601 acaccaattt cgtccacaga catcaacatc ttatcgtcct tatgacgtaa gccatgacgt
      661 ctagtgggag ccaccactaa aacatttgaa gataagataa taatgttgaa gataagaccg
      721 acagccactt gtgtgaagca tgtgaagccg gtccctccac taagaaaatt agtgaagcat
      781 cttccagtgg tccctccact cacagctcaa tcagtgagca acaggacgaa ggaagctttg
      841 tcaaaagcta aaaaagatga tgcatcccac aagaatttcc ttatataagg aacacaaatc
      901 agaaggtact cgagaccctg cagtccggca aaaaagggca aggtgtcacc accctgccct
      961 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
     1021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1081 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1141 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
     1201 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1261 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1321 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1381 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1441 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1501 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1561 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1621 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1681 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1741 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1801 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1861 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1921 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     1981 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     2041 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     2101 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     2161 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     2221 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     2281 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     2341 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     2401 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     2461 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcat
//
