LOCUS       sequence-299292-        2337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0120
FEATURES             Location/Qualifiers
     source          1..2337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     408..427
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     655..673
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(677..720)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1033..1090
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1114..1131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1285..1873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1365..1384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2056..2150)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2171..2337,1..493))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
       61 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
      121 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
      181 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
      241 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
      301 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
      361 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
      421 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
      481 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
      541 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact
      601 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      661 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
      721 tggaattcgg tctcaggaga gcggagaatt aagggagtca cgttatgacc cccgccgatg
      781 acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact
      841 cagccgcggg tttctggagt ttaaaccatt attgcgaatg agctaagcac atacgtcagc
      901 gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca
      961 ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac tctcatactc
     1021 gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
     1081 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
     1141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1261 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
     1321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1921 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1981 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2041 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2101 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2161 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     2221 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     2281 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagt
//
