LOCUS       sequence-299293-        2337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0119
FEATURES             Location/Qualifiers
     source          1..2337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     411..430
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     658..676
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(680..723)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1036..1093
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1117..1134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1288..1876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1368..1387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2059..2153)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2174..2337,1..496))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
       61 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      121 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      181 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
      241 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
      301 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
      361 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
      421 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
      481 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
      541 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
      601 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
      661 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
      721 ttctggaatt cggtctcagg agagcggaga attaagggag tcacgttatg acccccgccg
      781 atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc
      841 actcagccgc gggtttctgg agtttaaacc attattgcga tcactatcag ccgttcaaaa
      901 gtcgcctaag gatgagctaa gcacatacgt cagtagcaaa tatttcttgt caaaaatgct
      961 ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt cactctcata
     1021 ctcgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     1081 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     1141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1261 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2041 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     2101 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     2161 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     2221 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     2281 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaaga
//
