LOCUS       sequence-299295-        2477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0280
FEATURES             Location/Qualifiers
     source          1..2477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     277..296
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     524..542
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(546..589)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     964..984
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        973..1019
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1042..1099
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1123..1140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1294..1882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1374..1393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2065..2159)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2180..2477,1..362))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
       61 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
      121 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
      181 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      241 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      301 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      361 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
      421 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc
      481 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
      541 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcggt
      601 ctcaggaggt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
      661 cagtctcaga agatcaaagg gctattgaga caggataatt tcgggaaacc tcctcggatt
      721 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta
      781 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg
      841 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac
      901 gtctacaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
      961 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     1021 acgctactcg agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
     1081 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
     1141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1261 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     1321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1921 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1981 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2041 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     2101 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     2161 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     2221 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     2281 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     2341 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     2401 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     2461 aacacgccac atcttgc
//
