LOCUS       sequence-299299-        2472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0282
FEATURES             Location/Qualifiers
     source          1..2472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     294..313
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     541..559
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(563..606)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     976..996
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        985..1031
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1054..1111
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1135..1152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1306..1894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1386..1405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2077..2171)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2192..2472,1..379))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
       61 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
      121 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
      181 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
      241 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
      301 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
      361 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
      421 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
      481 tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
      541 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
      601 gatttctgga attcggtctc aggaggtcaa catggtggag cacgacactc tggtctactc
      661 caaaaatgtc aaagatacag tctcagaaga tcaaagggct attgagactt ttcaacaaag
      721 gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcgaaaagga
      781 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc
      841 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
      901 ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga
      961 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1021 tttggagagg acacgctact cgagaccctg cagtccggca aaaaagggca aggtgtcacc
     1081 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
     1141 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1201 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1261 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
     1321 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1381 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1441 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1501 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1561 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1621 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1681 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1741 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1801 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1861 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1921 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1981 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2041 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     2101 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     2161 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
     2221 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     2281 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     2341 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     2401 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     2461 ggttttcacc gt
//
