LOCUS       sequence-299300-        2468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0283
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     287..306
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     534..552
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(556..599)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        677..1022
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     965..985
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1043..1100
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1124..1141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1295..1883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1375..1394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2066..2160)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2181..2468,1..372))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
       61 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
      121 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
      181 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
      241 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
      301 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
      361 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
      421 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc
      481 gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      541 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
      601 ggaattcggt ctcaggaggt caacatggtg gagcacgaca ctctggtcta ctccaaaaat
      661 gtcaaagata cagtcttgag acttttcaac aaaggataat ttcgggaaac ctcctcggat
      721 tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct
      781 acaaatgcca tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg
      841 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca
      901 cgtctacaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat gacgcacaat
      961 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     1021 cacgctactc gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt
     1081 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc
     1141 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1201 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1261 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg
     1321 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1741 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1801 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1921 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2041 cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga
     2101 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     2161 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     2221 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     2281 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     2341 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     2401 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     2461 taacacgc
//
