LOCUS       sequence-299301-        2474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0278
FEATURES             Location/Qualifiers
     source          1..2474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..285
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     513..531
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(535..578)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     950..970
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        959..1005
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1028..1085
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1109..1126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1280..1868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1360..1379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2051..2145)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2166..2474,1..351))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
       61 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      121 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      181 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      241 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      301 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      361 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      421 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag
      481 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
      541 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcggtc tcaggaggtc
      601 aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa
      661 gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc
      721 cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaaaa tgccatcatt
      781 gcgataaagg aaaggctatc attcaagatc tctctgccga cagtggtccc aaagatggac
      841 ccccacccac gaggagcatc gtggaaaaag aagaggttcc aaccacgtct acaaagcaag
      901 tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      961 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tactcgagac
     1021 cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
     1081 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1261 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
     1321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1681 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1921 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1981 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     2041 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     2101 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     2161 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     2221 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     2281 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     2341 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     2401 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     2461 tgcgaatata tgtg
//
