LOCUS       sequence-299302-        2471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0284
FEATURES             Location/Qualifiers
     source          1..2471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     260..279
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     507..525
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(529..572)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        653..998
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     941..961
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1019..1076
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1100..1117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1271..1859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1351..1370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2042..2136)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2157..2471,1..345))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
       61 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
      121 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
      181 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
      241 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
      301 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
      361 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
      421 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
      481 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
      541 aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcagga ggtcaacatg
      601 gtggagcacg acactctggt ctactcccag tctcagaaga tcaaagggct attgagactt
      661 ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
      721 tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
      781 gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga cccccaccca
      841 cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat
      901 gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
      961 cctctatata aggaagttca tttcatttgg agaggacacg ctactcgaga ccctgcagtc
     1021 cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
     1081 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1261 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1681 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg
     2041 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     2101 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
     2161 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     2221 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     2281 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     2341 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     2401 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     2461 atgtgtagaa a
//
