LOCUS       sequence-299306-        2506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0289
FEATURES             Location/Qualifiers
     source          1..2506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     546..565
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     793..811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(815..858)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1340..1397
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1421..1438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1592..2180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1672..1691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2363..2457)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2478..2506,1..631))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
       61 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
      121 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
      181 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
      241 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
      301 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
      361 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
      421 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
      481 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
      541 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
      601 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
      661 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      721 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
      781 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
      841 ttcgctaagg atgatttctg gaattcggtc tcaggagtgg aagattcaga aaatctccat
      901 caacaaataa tccaagtaag gattaatgga ttgatcaaca tccttaccgc tatgggtaag
      961 attgatgaaa agtcaaaaac aaaaatcaat tatgcacacc agcatgtgtt gatcaccagc
     1021 tattgtggga caccaatttc gtccacagac atcaacatct tatcgtcctt tgaagataag
     1081 ataataatgt tgaagataag agtgggagcc accactaaaa cattgctttg tcaaaagcta
     1141 aaaaagatga tgcccgagaa gcatgtgaag ccggtccctc cactaagaaa attagtgaag
     1201 catcttccag tggtccctcc actcacagct caatcagtga gcaacaggac gaaggaaatg
     1261 acgtaagcca tgacgtctaa tcccacaaga atttccttat ataaggaaca caaatcagaa
     1321 ggtactcgag accctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt
     1381 ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc
     1441 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc
     1621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2341 ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     2401 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     2461 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagt
//
