LOCUS       sequence-299307-        2501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0286
FEATURES             Location/Qualifiers
     source          1..2501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     155..173
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(177..220)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      697..754
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(778..795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(949..1537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1029..1048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1720..1814)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1835..2494)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2409..2428
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
       61 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta
      121 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
      181 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg tctcaggagt
      241 ggaagattca gaaaatctcc atcaacaaat aatccaagta aggattaatg gattgatcaa
      301 caagattgat gaaaagtcaa aaacaaaaat caattatgca caccagcatg tgttgatcac
      361 cagctattgt gggacaccaa tttcgtccac agacatcaac atcttatcgt cctttgaaga
      421 taagataata atgttgaaga taagagtggg agccaccact aaaacattgc tttgtcaaaa
      481 gctaaaaaag atgatgcccg acagccactt gtgtgaagca tgtgaagccg gtccctccac
      541 taagaaaatt agtgaagcat cttccagtgg tccctccact cacagctcaa tcagtgagca
      601 acaggacgaa ggaaatgacg taagccatga cgtctaatcc cacaagaatt tccttatata
      661 aggaacacaa atcagaaggt actcgagacc ctgcagtccg gcaaaaaagg gcaaggtgtc
      721 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      781 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      841 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      901 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      961 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1021 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1081 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1141 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1201 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1261 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1321 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1381 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1441 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1501 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1561 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1621 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1681 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     1741 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1801 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     1861 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1921 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1981 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     2041 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     2101 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     2161 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     2221 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     2281 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     2341 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     2401 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2461 caacggtggt atatccagtg atttttttct ccattttagc t
//
