LOCUS       sequence-299311-        2504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0287
FEATURES             Location/Qualifiers
     source          1..2504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..297
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     525..543
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(547..590)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1070..1127
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1151..1168)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1322..1910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1402..1421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2093..2187)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2208..2504,1..363))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
       61 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
      121 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
      181 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
      241 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      301 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
      361 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
      421 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac
      481 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
      541 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg
      601 tctcaggagt ggaagattca gaaaatctcc atcaacaaat aatccaagta aggattaatg
      661 gattgatcaa catccttacc gctatgggta agattgatga aaagtcaaaa acaaaaatca
      721 attatgcaca ccagcatgtg ttgatcacca gctattgtgg gacaccaatt tcgtccacag
      781 acatcaacat cttatcgtcc tttgaagata agataataat gttgaagata agagtgggag
      841 ccaccactaa aacattgctt tgtcaaaagc taaaaaagat gatgcccgac agccacttgt
      901 gtgaagcatg tgaagccggt ccctccacta agaaaattag tgaagcatct tctcacagct
      961 caatcagtga gcaacaggac gaaggaaatg acgtaagcca tgacgtctaa tcccacaaga
     1021 atttccttat ataaggaaca caaatcagaa ggtactcgag accctgcagt ccggcaaaaa
     1081 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
     1141 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1201 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1261 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1321 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1381 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1441 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1501 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1561 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1621 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1681 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1741 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1801 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1861 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1921 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1981 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2041 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac
     2101 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     2161 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     2221 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     2281 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     2341 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     2401 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     2461 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttg
//
