LOCUS       sequence-299330-        2939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses tagRFP fluorescent protein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Matsumura I
  TITLE     Methylase-assisted subcloning for high throughput BioBrick assembly.
  JOURNAL   PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717/peerj.9841. eCollection 
            2020.
  PUBMED    32974095
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2020-09-11"; volume: "8:e9841. doi"; pages: " 
            10.7717/peerj.9841. eCollection 2020"
COMMENT     Sequence Label: Prham-tagRFP-IMBB2.4-pUC57-mini
FEATURES             Location/Qualifiers
     source          1..2939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        156..274
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     RBS             300..322
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1029..1049
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     rep_origin      complement(1148..1736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1228..1247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1907..2698,2699..2767))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2530..2549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2768..2872)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(2939,1..21)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aattcgcggc cgcttctaga ggatgtcgtc aacacggcga aatagtaatc acgaggtcag
       61 gttcttacct taaattttcg acggaaaacc acgtaaaaaa cgtcgatttt tcaagataca
      121 gcgtgaattt tcaggaaatg cggtgagcat cacatcacca caattcagca aattgtgaac
      181 atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag taacgagaag
      241 gtcgcgaatt gaggcgcttt ttagactggt cgtaatgaaa ttcagctact agaaataatt
      301 ttgtttaact ttaagaagga gatatacata tgtcagaact tatcaaggaa aatatgcaca
      361 tgaaattgta catggaagga actgtcaata atcaccactt taaatgtacc tcagaaggag
      421 aaggaaaacc gtatgaaggt actcaaacca tgcgtattaa ggttgtcgaa ggtggaccac
      481 ttccttttgc ttttgatatt cttgcaacat cttttatgta cggatcacgt acctttatca
      541 atcatacaca aggtatccca gatttcttta aacagtcttt tcctgaagga tttacatggg
      601 aacgtgttac aacttatgaa gatggtggag tcttgacagc tactcaagat acatctttgc
      661 aagatggttg tttgatctac aatgtcaaga tccgtggagt aaattttcca tctaatggtc
      721 ctgttatgca gaaaaagact cttggatggg aagccaatac cgaaatgttg tatcctgctg
      781 atggtggact cgagggtcgt tcagatatgg cattgaaact tgttggtgga ggtcatttga
      841 tctgtaattt taagaccaca taccgtagta aaaagccagc aaagaatctt aagatgcctg
      901 gtgtatacta cgtcgatcat cgtttggaac gtatcaaaga agccgataag gaaacttatg
      961 ttgaacaaca cgaagtcgcc gtagcacgtt actgtgattt gcctagtaaa ttgggacata
     1021 aataagctta ctagtagcgg ccgctgcagc atcgatgttt aaacgagctc atgcatatcg
     1081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1561 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1801 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1861 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1921 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1981 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2041 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2101 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2161 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2221 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2281 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2341 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2401 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2461 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2521 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2581 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2641 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2701 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2761 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2821 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2881 cccgaaaagt gccacctgac gtcccaatga tcaattgggt accttaatta agacgtcgg
//
