LOCUS       sequence-299361-        9464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma
            PylRS for ochre suppression in transient or stable, 
            piggybac-mediated, integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ
  TITLE     Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal
            Labeling and Crosslinking of Proteins on Live Mammalian Cells.
  JOURNAL   Cell Rep. 2020 Jun 23;31(12):107811. doi: 
            10.1016/j.celrep.2020.107811.
  PUBMED    32579937
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-06-23"; volume: "31(12):107811. doi"; pages: " 
            10.1016/j.celrep.2020.107811"
COMMENT     Sequence Label: pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS
FEATURES             Location/Qualifiers
     source          1..9464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   288..350
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(871..1119)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(1209..1457)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(1547..1795)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(1885..2133)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        2196..2407
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2420..2688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2720..2729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2729..2752
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4136..4710,4711..4713,4714..4722)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4303..4320)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4530..4549
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4735..5334
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4735..4754)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5231..5251
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5394..5414)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5449..5570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5486..5505)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5540..5559
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6332..6366
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6857..6873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6857..6873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6870..6892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6881..6897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6905..6911,6912..6929,6930..6935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6950..6971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7088..7105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7259..7847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7339..7358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8018..8809,8810..8878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8641..8660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8879..8983)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9009..9464)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9146..9167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9358..9377
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg
      181 tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg
      241 agcagatgaa gatgctcgac acgctgcaga acacgcagct agattaaccc tagaaagata
      301 atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg tgttttatcg
      361 gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca
      421 cttacatact aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa
      481 aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttatgagg gacagccccc
      541 ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg
      601 cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg
      661 acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct
      721 ctttgagcct gcagacacct ggggggaatt cgggaaaagg cctccacggc caaccaatga
      781 gacgtaaaaa ccgcacttgt ccggaaaccc cgggaatcga acccggctga acggatttaa
      841 agtccgttcg gtctccctga ccaggtttcc ggtgtttcgt cctttccaca agatatataa
      901 agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca
      961 taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac
     1021 taatatcttt gtgtttacag tcaaattaat tctaattatc tctctaacag ccttgtatcg
     1081 tatatgcaaa tatgaaggaa tcatgggaaa taggccctcg caaaaaaacc gcacttgtcc
     1141 ggaaaccccg ggaatcgaac ccggctgaac ggatttaaag tccgttcggt ctccctgacc
     1201 aggtttccgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt
     1261 caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac
     1321 ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc
     1381 aaattaattc taattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc
     1441 atgggaaata ggccctccaa gaaaaaccgc acttgtccgg aaaccccggg aatcgaaccc
     1501 ggctgaacgg atttaaagtc cgttcggtct ccctgaccag gtttccggtg tttcgtcctt
     1561 tccacaagat atataaagcc aagaaatcga aatactttca agttacggta agcatatgat
     1621 agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac
     1681 gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcta attatctctc
     1741 taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctccgata
     1801 aaaaccgcac ttgtccggaa accccgggaa tcgaacccgg ctgaacggat ttaaagtccg
     1861 ttcggtctcc ctgaccaggt ttccggtgtt tcgtcctttc cacaagatat ataaagccaa
     1921 gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa aacataattt
     1981 taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat
     2041 ctttgtgttt acagtcaaat taattctaat tatctctcta acagccttgt atcgtatatg
     2101 caaatatgaa ggaatcatgg gaaataggcc ctcctccaag cctagggcca ctagtgctct
     2161 agaaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     2221 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     2281 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     2341 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     2401 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     2461 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     2521 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     2581 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     2641 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     2701 ggcgcctact ctagagctag ccaccatgga ctacaaggac gacgacgaca agatggacaa
     2761 gaagcccctg aacaccctga tcagcgccac aggactgtgg atgtccagaa ccggcaccat
     2821 ccacaagatc aagcaccacg aggtgtcccg gtccaaaatc tacatcgaga tggcctgcgg
     2881 cgatcacctg gtcgtcaaca acagcagaag cagccggaca gccagagccc tgcggcacca
     2941 caagtacaga aagacctgca agcggtgcag agtgtccgac gaggacctga acaagttcct
     3001 gaccaaggcc aacgaggacc agaccagcgt gaaagtgaag gtggtgtccg cccccacccg
     3061 gaccaagaaa gccatgccca agagcgtggc cagagccccc aagcccctgg aaaacaccga
     3121 agccgctcag gcccagccca gcggcagcaa gttcagcccc gccatccccg tgtctaccca
     3181 ggaaagcgtc agcgtccccg ccagcgtgtc caccagcatc tctagcatct caaccggcgc
     3241 cacagcttct gccctggtca agggcaacac caaccccatc accagcatgt ctgcccctgt
     3301 gcaggcctct gccccagccc tgaccaagtc ccagaccgac cggctggaag tgctcctgaa
     3361 ccccaaggac gagatcagcc tgaacagcgg caagcccttc cgggagctgg aaagcgagct
     3421 gctgagccgg cggaagaagg acctccagca aatctacgcc gaggaacggg agaactacct
     3481 gggcaagctg gaaagagaga tcacccggtt cttcgtggac cggggcttcc tggaaatcaa
     3541 gagccccatc ctgatccccc tggagtacat cgagcggatg ggcatcgaca acgacaccga
     3601 gctgagcaag cagattttcc gggtggacaa gaacttctgc ctgcggccca tgctggcccc
     3661 caacctgtac aactacctgc ggaaactgga tcgcgctctg cccgacccca tcaagatttt
     3721 cgagatcggc ccctgctacc ggaaagagag cgacggcaaa gagcacctgg aagagtttac
     3781 aatgctgaac ttttgccaga tgggcagcgg ctgcaccaga gagaacctgg aatccatcat
     3841 caccgacttt ctgaaccacc tggggatcga cttcaagatc gtgggcgaca gctgcatggt
     3901 gtacggcgac accctggacg tgatgcacgg cgacctggaa ctgtctagcg ccgtcgtggg
     3961 acccatccct ctggaccggg agtggggcat cgataagccc tggatcggag ccggcttcgg
     4021 cctggaacgg ctgctgaaag tcaagcacga ctttaagaac atcaagcggg ctgccagaag
     4081 cgagagctac tacaacggca tcagcaccaa cctgtgatga ggatccgcgg ccgcgcccct
     4141 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     4201 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     4261 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4321 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4381 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4441 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4501 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     4561 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     4621 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     4681 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggcgtc cggaatgacc
     4741 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     4801 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     4861 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4921 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4981 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5041 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5101 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     5161 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5221 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5281 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac
     5341 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5401 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgttaa cttgtttatt
     5461 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5521 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5581 aattgactca aatgatgtca attagtctat cagaagctat ctggtctccc ttccggggga
     5641 caagacatcc ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct
     5701 tgctctggtc aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa
     5761 acagtggcag ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt
     5821 atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt
     5881 cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg
     5941 ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc
     6001 cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat
     6061 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca
     6121 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt
     6181 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc
     6241 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta
     6301 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt
     6361 ctagggttaa tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat
     6421 cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag
     6481 gttgaagcgg catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga
     6541 aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa
     6601 ctgttcgttc aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg
     6661 atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt
     6721 gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg
     6781 tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc
     6841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7141 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7201 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7261 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7321 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7381 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7441 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7501 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7561 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7621 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7681 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7741 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7801 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7861 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7921 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7981 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8041 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8101 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8161 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8221 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8281 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8341 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8401 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8461 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8521 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8581 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8641 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8701 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8761 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8821 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8881 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8941 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9001 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     9061 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     9121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     9181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     9241 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     9301 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     9361 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     9421 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgt
//
