LOCUS       sequence-299384-        2184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0374
FEATURES             Location/Qualifiers
     source          1..2184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(13..56)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      216..273
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(297..314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(468..1056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(548..567)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1239..1333)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1354..2013)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1928..1947
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     join(2175..2184,1..9)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
       61 attcggtctc aggagggagg cgtgtcgttt tagtgaggat cgaaaggaca gtaatcgaaa
      121 ggacagtaat cgaaaggaca gtactatata aggttttgct attcattgaa agcagtagtg
      181 actgatttgt atataatgta ctcgagaccc tgcagtccgg caaaaaaggg caaggtgtca
      241 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg
      301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
      481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      661 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      901 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1141 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1201 atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc
     1261 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1321 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     1381 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1441 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1501 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1561 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     1621 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1681 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1741 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1801 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1861 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1921 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1981 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     2041 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     2101 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga
     2161 cattaaccta taaaaatagg cgta
//
