LOCUS       sequence-299393-        2184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0377
FEATURES             Location/Qualifiers
     source          1..2184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..118
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(122..165)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      325..382
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(406..423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(577..1165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(657..676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1348..1442)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1463..2122)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2037..2056
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
       61 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
      121 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcggtctca
      181 ggagggaggc gtgtcgtttt agtgaggtgg tggagcacga catggtggag cacgacatgg
      241 tggagcacga cactatataa ggttttgcta ttcattgaaa gcagtagtga ctgatttgta
      301 tataatgtac tcgagaccct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc
      361 tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc
      421 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      481 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      541 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga
      601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1081 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1141 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1201 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1321 aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     1381 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     1441 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     1501 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1561 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1621 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     1681 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     1741 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     1801 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     1861 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     1921 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     1981 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     2041 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     2101 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     2161 aaaatacgcc cggtagtgat ctta
//
