LOCUS       sequence-299398-        2187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0431
FEATURES             Location/Qualifiers
     source          1..2187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     99..118
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     346..364
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(368..411)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      574..631
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(655..672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(826..1414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(906..925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1597..1691)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1712..2187,1..184))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
       61 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
      121 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
      181 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
      241 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca
      301 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      361 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
      421 gtctcaggag ggaggcgtgt cgttttagtg aggttctctc tgccgacagt ggtcccaaaa
      481 accacgtcta caaagcaagt ggatctatat aaggttttgc tattcattga aagcagtagt
      541 gactgatttg tatatatact cgagaccctg cagtccggca aaaaagggca aggtgtcacc
      601 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
      661 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
      841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1261 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1561 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     1621 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1681 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
     1741 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1801 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1861 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1921 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1981 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     2041 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     2101 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     2161 gatgagcatt catcaggcgg gcaagaa
//
