LOCUS       sequence-299401-        2213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0437
FEATURES             Location/Qualifiers
     source          1..2213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..70
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     298..316
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(320..363)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      552..609
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(633..650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(804..1392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(884..903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1575..1669)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1690..2213,1..136))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
       61 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
      121 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
      181 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
      241 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
      301 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
      361 ttctggaatt cggtctcagg agggaggcgt gtcgttttag tgaggaagat tgatgaaaag
      421 tcaaaaacaa aaatcaatta tgcacaccag catgtgttga tcaccagctc agccacttgt
      481 gtctatataa ggttttgcta ttcattgaaa gcagtagtga ctgatttgta tatatactcg
      541 agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
      601 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      781 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1201 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1441 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1501 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1561 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1621 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1681 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     1741 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1801 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1861 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     1921 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     1981 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     2041 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     2101 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     2161 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tct
//
