LOCUS       sequence-299403-        2186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0434
FEATURES             Location/Qualifiers
     source          1..2186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..21
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     249..267
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(271..314)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      476..533
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(557..574)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(728..1316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(808..827)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1499..1593)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1614..2186,1..87))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
       61 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
      121 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      181 gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa
      241 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
      301 ctaaggatga tttctggaat tcggtctcag gagggaggcg tgtcgtttta gtgaggtggt
      361 ggagcacgac aaaaaatgtc aaagatatca gaagatcaaa gggctactat ataaggtttt
      421 gctattcatt gaaagcagta gtgactgatt tgtatatata ctcgagaccc tgcagtccgg
      481 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      541 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      601 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      721 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      781 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      841 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      901 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      961 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1021 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1081 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1141 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1201 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
     1501 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1561 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1621 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1681 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1741 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1801 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     1861 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1921 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1981 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     2041 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     2101 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     2161 tgaacggtct ggttataggt acattg
//
