LOCUS       sequence-299410-        2362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0313
FEATURES             Location/Qualifiers
     source          1..2362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     264..283
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     511..529
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(533..576)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    579..600
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      914..971
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(995..1012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1166..1754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1246..1265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1937..2031)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2052..2362,1..349))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt tcagtttgct
       61 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
      121 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
      181 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
      241 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
      301 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
      361 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      421 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa
      481 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
      541 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
      601 cgatgagtgg tctcgggagg gaggcgtgtc gttttagtga ggtcattcaa gatctctctg
      661 ccgacagtgg tcccaaagat ggaccccaag aggttccaac cacgtctaca aagcaagtgg
      721 attgatgtga catctccact gacgtaaggg atgacgcaca atcccactat tttattgggt
      781 acccgaataa ctcagcatct tttgctaaat tctatcgtgg ctatataagg ttttgctatt
      841 cattgaaagc agtagtgact gatttgtata tatacttgag accactcatc gcgactagta
      901 gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa
      961 aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg
     1021 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1081 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1141 gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca
     1201 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1261 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1321 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1381 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1441 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1501 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1561 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1621 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1681 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1741 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1801 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1861 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1921 acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     1981 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     2041 tcgatatcaa attacgcccc gccctgccac tcgtcgcagt actgttgtaa ttcattaagc
     2101 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     2161 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     2221 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgatacg
     2281 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     2341 acatcttgcg aatatatgtg ta
//
