LOCUS       sequence-299413-        2306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0209
FEATURES             Location/Qualifiers
     source          1..2306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     182..201
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     429..447
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(451..494)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      776..833
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(857..874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1028..1616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1108..1127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1799..1893)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1914..2306,1..267))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
       61 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
      121 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
      181 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
      241 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
      301 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      361 gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa
      421 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
      481 ctaaggatga tttctggaat tcggtctcag gagggaggcg tgtcgtttta gtgaggtatc
      541 gtcctttgaa gataagataa taatgttgaa gataagagtg ggagccacca ctaaaacatt
      601 gctttgtcaa aagctaaaaa agatgatgcc cgacagccag acgaaggaaa tgacgtaagc
      661 catgacgtct aatcccacaa gtactgttgg actctgaaga agatacctat ataaggtttt
      721 gctattcatt gaaagcagta gtgactgatt tgtatatata ctcgagaccc tgcagtccgg
      781 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      841 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1021 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1441 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
     1801 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1861 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1921 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1981 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     2041 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     2101 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     2161 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     2221 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     2281 tgctcatgga aaacggtgta acaagg
//
