LOCUS       sequence-299416-        2271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0311
FEATURES             Location/Qualifiers
     source          1..2271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     240..259
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     487..505
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(509..552)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    555..576
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      799..856
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(880..897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1051..1639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1131..1150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1822..1916)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1937..2271,1..325))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
       61 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      121 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
      181 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
      241 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
      301 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
      361 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
      421 gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
      481 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
      541 aaggatgatt tctggaattc gcggccgctt ctagagcgat gagtggtctc gggagggagg
      601 cgtgtcgttt tagtgaggct ggagtttaat gagctaagca catacgtcag aaaccattat
      661 tgcgcgttca aaagtcgcct aaggtcacta tcagctagca aatatctata taaggttttg
      721 ctattcattg aaagcagtag tgactgattt gtatatatac ttgagaccac tcatcgcgac
      781 tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
      841 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
      901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      961 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1021 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     1081 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1141 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1201 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1261 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1321 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1381 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1441 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1501 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1561 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1621 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1681 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1741 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1801 gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     1861 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     1921 cgagctcgat atcaaattac gccccgccct gccactcgtc gcagtactgt tgtaattcat
     1981 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     2041 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     2101 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     2161 atacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     2221 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg t
//
