LOCUS       sequence-29944-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Jelezcova E, Trivedi RN, Wang XH, Tang JB, Brown AR, Goellner EM, 
            Schamus S, Fornsaglio JL, Sobol RW
  TITLE     Parp1 activation in mouse embryonic fibroblasts promotes Pol 
            beta-dependent cellular hypersensitivity to alkylation damage.
  JOURNAL   Mutat Res. 2010 Apr 1;686(1-2):57-67. doi: 
            10.1016/j.mrfmmm.2010.01.016. Epub 2010 Jan 22.
  PUBMED    20096707
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mutat 
            Res."; date: "2010-04-1"; volume: "686(1-2):57-67. doi"; pages: " 
            10.1016/j.mrfmmm.2010.01.016. Epub 2010 Jan 22"
COMMENT     Sequence Label: pIRES.Puro.mBeta
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agcttggtac cgagctcgga tcgatatcaa caagtttgta caaaaaagca ggctccgcgg
      121 ccgccccctt caccatgagc aaacgcaagg cgccgcagga gaccctcaac ggcggcatca
      181 cggacatgct cgtggaactc gcaaactttg agaagaacgt gagccaggcg atccacaagt
      241 acaatgcgta cagaaaagcg gcatctgtga tagccaagta cccacacaaa atcaagagcg
      301 gagcggaagc taagaaactg ccaggagtag gaacaaaaat tgctgaaaag attgatgaat
      361 ttttagcaac tggaaaattg cgtaaactgg aaaagattcg tcaagatgat acaagttcat
      421 ccatcaattt cctgactcga gttactggca ttggaccatc tgctgcaagg aagtttgtag
      481 atgaaggaat taagacatta gaagatctca ggaaaaatga agataaactg aaccatcatc
      541 aacgaattgg gctgaaatat ttcgaggact ttgaaaagag aattcctcgt gaggaaatgc
      601 tgcagatgca ggatattgta cttaatgaaa ttaaaaaagt ggactctgag tacattgcta
      661 cagtctgtgg cagtttcaga agaggcgcag agtcgagtgg agacatggat gtcctgctga
      721 cccacccaa
//
