LOCUS       sequence-299448-        2326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0417
FEATURES             Location/Qualifiers
     source          1..2326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     208..227
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     455..473
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(477..520)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      822..879
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(903..920)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1074..1662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1154..1173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1845..1939)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1960..2326,1..293))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
       61 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      121 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      181 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      241 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
      301 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
      361 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta
      421 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
      481 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg tctcaggagg
      541 gaggcgtgtc gttttagtga ggattgcgat aaaggaaagg catgaagata agataataat
      601 gttgaagata agactgctag gaggacctga cgtaagggat gacgcacagg aaagattgat
      661 gaaaagtcaa aaacaaaaat caattatgta atccttaccg ctatgggtaa gattacctca
      721 catgtaggct atcttttcaa caaggctcac tgctatataa ggttttgcta ttcattgaaa
      781 gcagtagtga ctgatttgta tatatactcg agaccctgca gtccggcaaa aaagggcaag
      841 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct
      901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      961 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1021 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
     1081 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1261 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1381 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1441 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1501 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1681 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1741 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1801 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa
     1861 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     1921 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     1981 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     2041 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     2101 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     2161 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     2221 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     2281 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtt
//
