LOCUS       sequence-299456-        2310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0425
FEATURES             Location/Qualifiers
     source          1..2310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..183
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     411..429
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(433..476)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      762..819
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(843..860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1014..1602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1094..1113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1785..1879)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1900..2310,1..249))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
       61 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
      121 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
      181 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
      241 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
      301 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag
      361 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
      421 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
      481 attcggtctc aggagggagg cgtgtcgttt tagtgagggt gggagccacc atatcagctt
      541 agccagtggt ccctccacat gctgattttc aacaaaaact ggtattatga cccccgccga
      601 tgacgcggga aaaactggtg gtggagcacg acaacttgtg tacagtcact atcagcgcta
      661 tcatgacgta agccatgacg tctacacatg tactatataa ggttttgcta ttcattgaaa
      721 gcagtagtga ctgatttgta tatatactcg agaccctgca gtccggcaaa aaagggcaag
      781 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct
      841 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      901 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      961 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
     1021 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1081 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1141 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1201 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1261 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1321 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1381 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1441 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1501 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1561 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1621 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1681 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1741 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa
     1801 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     1861 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     1921 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     1981 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     2041 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     2101 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     2161 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     2221 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     2281 ggtgtaacaa gggtgaacac tatcccatat
//
