LOCUS       sequence-299460-        2419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0429
FEATURES             Location/Qualifiers
     source          1..2419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..173
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     401..419
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(423..466)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      861..918
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(942..959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1113..1701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1193..1212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1884..1978)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1999..2419,1..239))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
       61 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
      121 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
      181 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
      241 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
      301 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
      361 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
      421 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc
      481 aggagggagg cgtgtcgttt tagtgaggaa gattgatgaa aagtcaaaaa caaaaatcaa
      541 ttatcactgc taggaaacca ttattgcgac aaaactggta ctttcagaag atcaaagggc
      601 tataggagga ctccatcaac aaataatcca agtaagctag gaggaccgat gctgatgctt
      661 tgtcaaaagc taaaaaagat gatgcctgct aggaggaccg atgccaaata tttcttgtgc
      721 actggtggag cacgacagac ctctacaaac tgacgtaagg gatgacgcac acctaaccac
      781 gtctacaacc tctatataag gttttgctat tcattgaaag cagtagtgac tgatttgtat
      841 atatactcga gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
      901 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
      961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1081 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     1141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1201 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1261 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1321 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1381 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1441 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1501 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1561 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1621 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1681 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1741 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1861 tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     1921 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     1981 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     2041 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     2101 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     2161 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     2221 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     2281 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     2341 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     2401 atcccatatc accagctca
//
