LOCUS       sequence-299461-        2279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0430
FEATURES             Location/Qualifiers
     source          1..2279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     139..158
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     386..404
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(408..451)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      706..763
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(787..804)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(958..1546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1038..1057)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1729..1823)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1844..2279,1..224))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
       61 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      121 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
      181 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
      241 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
      301 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat
      361 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
      421 aaaatcctta gctttcgcta aggatgattt ctggaattcg gtctcaggag ggaggcgtgt
      481 cgttttagtg aggtgaagat aagataataa tgttgaagat aagatatcct taccgctatg
      541 ggtaagattc atgtaggctt tatgaccccc gccgatgacg cgggagtctc tctgccgaca
      601 gtggtcccaa atgctgacgt aagggatgac gcacatctat ataaggtttt gctattcatt
      661 gaaagcagta gtgactgatt tgtatatata ctcgagaccc tgcagtccgg caaaaaaggg
      721 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca
      781 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      841 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      901 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      961 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1021 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1081 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1141 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1201 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1261 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1321 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1381 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1441 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1501 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1561 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1621 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1681 aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat
     1741 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     1801 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     1861 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     1921 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     1981 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     2041 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     2101 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     2161 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     2221 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcc
//
