LOCUS       sequence-299479-        2558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0178
FEATURES             Location/Qualifiers
     source          1..2558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(764..781)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(874..1462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(954..973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1636..2430)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2431..2535)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatt cggtctcagg agagcggaga attaagggag tcacgttatg acccccgccg
      421 atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc
      481 actcagccgc gggtttctgg agtttaaaac cattattgcg cgttcaaaag tcgcctaagg
      541 tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc
      601 cctcggtatc caattagagt ctcatattca ctctcatact cgagaccctc tgggcctcat
      661 gggccttcct ttcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaac
      721 atggtcatag ctgtttcctt gcgtattggg cgctctccgc ttcctcgctc actgactcgc
      781 tgcgctcggt cgttcgggta aagcctgggg tgcctaatga gcaaaaggcc agcaaaaggc
      841 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      901 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      961 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1021 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1081 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1141 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1201 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1261 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1321 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1381 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1441 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1501 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1561 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1621 ttggtctgac agttattaga aaaattcatc cagcagacga taaaacgcaa tacgctggct
     1681 atccggtgcc gcaatgccat acagcaccag aaaacgatcc gcccattcgc cgcccagttc
     1741 ttccgcaata tcacgggtgg ccagcgcaat atcctgataa cgatccgcca cgcccagacg
     1801 gccgcaatca ataaagccgc taaaacggcc attttccacc ataatgttcg gcaggcacgc
     1861 atcaccatgg gtcaccacca gatcttcgcc atccggcatg ctcgctttca gacgcgcaaa
     1921 cagctctgcc ggtgccaggc cctgatgttc ttcatccaga tcatcctgat ccaccaggcc
     1981 cgcttccata cgggtacgcg cacgttcaat acgatgtttc gcctgatgat caaacggaca
     2041 ggtcgccggg tccagggtat gcagacgacg catggcatcc gccataatgc tcactttttc
     2101 tgccggcgcc agatggctag acagcagatc ctgacccggc acttcgccca gcagcagcca
     2161 atcacggccc gcttcggtca ccacatccag caccgccgca cacggaacac cggtggtggc
     2221 cagccagctc agacgcgccg cttcatcctg cagctcgttc agcgcaccgc tcagatcggt
     2281 tttcacaaac agcaccggac gaccctgcgc gctcagacga aacaccgccg catcagagca
     2341 gccaatggtc tgctgcgccc aatcatagcc aaacagacgt tccacccacg ctgccgggct
     2401 acccgcatgc aggccatcct gttcaatcat actcttcctt tttcaatatt attgaagcat
     2461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2521 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
