LOCUS       sequence-299483-        2488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0182
FEATURES             Location/Qualifiers
     source          1..2488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(694..711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(804..1392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(884..903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1566..2360)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2361..2465)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctctcggt ctcaggaggg aggcgtgtcg ttttagtgag
      421 gatctctctg ccgacagtgg tcccaaaaac cacgtctaca aagcaagtgg atctgacgta
      481 agggatgacg cacactatat aaggttttgc tattcattga aagcagtagt gactgatttg
      541 tatataatgt actcgagacc ctggtacctg gagcacaaga ctggcctcat gggccttcct
      601 ttcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaac atggtcatag
      661 ctgtttcctt gcgtattggg cgctctccgc ttcctcgctc actgactcgc tgcgctcggt
      721 cgttcgggta aagcctgggg tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt
      781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1201 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1441 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1501 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1561 agttattaga aaaattcatc cagcagacga taaaacgcaa tacgctggct atccggtgcc
     1621 gcaatgccat acagcaccag aaaacgatcc gcccattcgc cgcccagttc ttccgcaata
     1681 tcacgggtgg ccagcgcaat atcctgataa cgatccgcca cgcccagacg gccgcaatca
     1741 ataaagccgc taaaacggcc attttccacc ataatgttcg gcaggcacgc atcaccatgg
     1801 gtcaccacca gatcttcgcc atccggcatg ctcgctttca gacgcgcaaa cagctctgcc
     1861 ggtgccaggc cctgatgttc ttcatccaga tcatcctgat ccaccaggcc cgcttccata
     1921 cgggtacgcg cacgttcaat acgatgtttc gcctgatgat caaacggaca ggtcgccggg
     1981 tccagggtat gcagacgacg catggcatcc gccataatgc tcactttttc tgccggcgcc
     2041 agatggctag acagcagatc ctgacccggc acttcgccca gcagcagcca atcacggccc
     2101 gcttcggtca ccacatccag caccgccgca cacggaacac cggtggtggc cagccagctc
     2161 agacgcgccg cttcatcctg cagctcgttc agcgcaccgc tcagatcggt tttcacaaac
     2221 agcaccggac gaccctgcgc gctcagacga aacaccgccg catcagagca gccaatggtc
     2281 tgctgcgccc aatcatagcc aaacagacgt tccacccacg ctgccgggct acccgcatgc
     2341 aggccatcct gttcaatcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2401 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2461 ccgcgcacat ttccccgaaa agtgccac
//
