LOCUS       sequence-299485-        2461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0184
FEATURES             Location/Qualifiers
     source          1..2461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(667..684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(777..1365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(857..876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1539..2333)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2334..2438)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctctcggt ctcaggaggg aggcgtgtcg ttttagtgag
      421 gatgagctaa gcacatacgt cagaaccatt attgcgtcac tatcagccta tataaggttt
      481 tgctattcat tgaaagcagt agtgactgat ttgtatataa tgtactcgag accctggtac
      541 ctggagcaca agactggcct catgggcctt cctttcactg cccgctttcc agtcgggaaa
      601 cctgtcgtgc cagctgcatt aacatggtca tagctgtttc cttgcgtatt gggcgctctc
      661 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa
      721 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      781 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      841 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      901 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      961 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1021 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1081 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1141 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1201 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1261 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1321 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1381 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1441 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1501 atctaaagta tatatgagta aacttggtct gacagttatt agaaaaattc atccagcaga
     1561 cgataaaacg caatacgctg gctatccggt gccgcaatgc catacagcac cagaaaacga
     1621 tccgcccatt cgccgcccag ttcttccgca atatcacggg tggccagcgc aatatcctga
     1681 taacgatccg ccacgcccag acggccgcaa tcaataaagc cgctaaaacg gccattttcc
     1741 accataatgt tcggcaggca cgcatcacca tgggtcacca ccagatcttc gccatccggc
     1801 atgctcgctt tcagacgcgc aaacagctct gccggtgcca ggccctgatg ttcttcatcc
     1861 agatcatcct gatccaccag gcccgcttcc atacgggtac gcgcacgttc aatacgatgt
     1921 ttcgcctgat gatcaaacgg acaggtcgcc gggtccaggg tatgcagacg acgcatggca
     1981 tccgccataa tgctcacttt ttctgccggc gccagatggc tagacagcag atcctgaccc
     2041 ggcacttcgc ccagcagcag ccaatcacgg cccgcttcgg tcaccacatc cagcaccgcc
     2101 gcacacggaa caccggtggt ggccagccag ctcagacgcg ccgcttcatc ctgcagctcg
     2161 ttcagcgcac cgctcagatc ggttttcaca aacagcaccg gacgaccctg cgcgctcaga
     2221 cgaaacaccg ccgcatcaga gcagccaatg gtctgctgcg cccaatcata gccaaacaga
     2281 cgttccaccc acgctgccgg gctacccgca tgcaggccat cctgttcaat catactcttc
     2341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2461 c
//
