LOCUS       sequence-299555-        9760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-GIGYF2 in mammalian cells, can be used to make 
            inducible cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Tollenaere MAX, Tiedje C, Rasmussen S, Nielsen JC, Vind AC, Blasius 
            M, Batth TS, Mailand N, Olsen JV, Gaestel M, Bekker-Jensen S
  TITLE     GIGYF1/2-Driven Cooperation between ZNF598 and TTP in 
            Posttranscriptional Regulation of Inflammatory Signaling.
  JOURNAL   Cell Rep. 2019 Mar 26;26(13):3511-3521.e4. doi: 
            10.1016/j.celrep.2019.03.006.
  PUBMED    30917308
REFERENCE   2  (bases 1 to 9760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.03"; journalName: "Cell Rep"; date: 
            "2019-03-26- 26"; volume: "26"; issue: "13"; pages: "3511-3521.e4"
COMMENT     Sequence Label: pcDNA4/TO/GFP-GIGYF2
FEATURES             Location/Qualifiers
     source          1..9760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(984..986,987..989,990..1697)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(984..986,987..989,990..1697)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1029..1050)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1290..1309)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1637..1658
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1716..1740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3819..3830
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5650..5674)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5768..5785)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5774..5998
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6044..6472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6131..6150)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6341..6362
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6481..6501)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6486..6815
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6666..6801
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6863..6910
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6929..7303
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7433..7554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7470..7489)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7524..7543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7603..7619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7603..7619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7616..7638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7627..7643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7651..7657,7658..7675,7676..7681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7696..7717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7834..7851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8005..8593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8085..8104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8764..9555,9556..9624))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9387..9406
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9625..9729)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatggtga gcaagggcga ggagctgttc accggggtgg
     1021 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1081 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1141 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1201 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1261 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1321 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1381 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1441 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1501 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1561 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     1621 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1681 gcatggacga gctgtacggt accgagctcg gatctacaag tttgtacaaa aaagcaggct
     1741 ccaccatggc agcggaaacg cagacactga actttgggcc tgaatggctc cgagctctgt
     1801 ccagtggtgg gagtattaca tcccctcctc tttctccagc attgccgaag tataaattag
     1861 cagattatcg ttacggcaga gaagaaatgt tagcactttt ccttaaagac aacaagatac
     1921 cttcagacct tctggataaa gaatttctgc ctatcctcca ggaggaaccc cttccaccat
     1981 tggctctggt accctttaca gaagaagaac agagaaactt ttccatgtct gtaaatagtg
     2041 ctgctgtcct gcgattgaca ggacgaggag gaggaggaac agtggtgggg gctcctagag
     2101 gtcgaagttc ttcaagaggg cgaggcagag gcagaggtga atgtggtttc taccaaagaa
     2161 gttttgatga agtagagggt gtttttggtc gaggaggtgg cagagaaatg catagatcgc
     2221 agagctggga ggaaaggggt gacagacgtt ttgaaaaacc aggacgaaaa gatgtaggga
     2281 gaccaaattt tgaggaaggt ggaccaacat cagtagggag aaagcatgaa tttatacgct
     2341 cagaaagtga aaattggcgc atctttagag aggaacaaaa tggagaagat gaagatggag
     2401 gttggcgact agctggatca aggagggatg gagagaggtg gcgacctcac agtcctgatg
     2461 gccctcgttc tgcaggctgg cgggaacaca tggaacgacg tcggaggttt gagtttgatt
     2521 ctcgagatag agatgatgaa cggggttacc gaagggttcg ctctggcagt gggagcatag
     2581 atgatgacag ggatagcttg cccgaatggt gcttagagga tgctgaagaa gaaatgggta
     2641 catttgactc atctggagca ttcctttctc taaaaaaagt acagaaagag cctattccag
     2701 aagagcagga gatggacttc cggcctgtgg acgaagggga ggagtgctct gactctgagg
     2761 gtagccataa tgaagaggcc aaagaacccg ataagacaaa taagaaagaa ggagagaaaa
     2821 cagatagagt aggagttgaa gctagtgagg aaactcccca gacctcatca tcatctgcta
     2881 gaccaggtac tccttcagac catcagtctc aggaagcatc acagtttgag aggaaagatg
     2941 aaccaaaaac tgagcaaacg gaaaaagctg aagaggagac tcggatggaa aatagtctac
     3001 cagccaaagt gcccagcaga ggggatgaaa tggttgctga tgtccagcag cccctgtcgc
     3061 agattccttc agatacagcc tctcctcttc tcatacttcc acctcctgtt cccaatccta
     3121 gtcctactct ccggccagtt gaaacaccag ttgtaggtgc tcctggtatg ggcagtgttt
     3181 ccacagaacc tgatgatgaa gaaggtctca aacatttgga gcagcaagct gagaaaatgg
     3241 tggcttatct ccaagacagt gcactagatg atgaaagatt ggcatcaaaa ctgcaagagc
     3301 acagagctaa aggagtgtcg attccattga tgcatgaagc aatgcagaag tggtattaca
     3361 aagatcctca gggagaaatt caaggtccct tcaataatca ggagatggca gaatggtttc
     3421 aggcgggcta ttttactatg tctttattgg tgaagagagc gtgtgatgaa agcttccaac
     3481 ctcttggcga tatcatgaaa atgtggggaa gggttccctt ttctccaggt ccggctcccc
     3541 ctcctcatat gggagagctg gaccaggaac gactgaccag gcagcaagaa ctcacagcct
     3601 tataccagat gcagcacctg cagtaccagc agtttttaat acaacaacaa tatgcacagg
     3661 ttttggccca acagcagaaa gcagcactgt cttcccagca gcagcagcag ttggcacttc
     3721 ttcttcaaca gtttcagacc ttgaagatga gaatatctga tcagaacatc attccctcag
     3781 taactaggtc tgtgtccgtg ccagatactg gctctatctg ggagcttcag ccaacagctt
     3841 cacagcctac agtttgggaa ggtggtagtg tatgggatct tcctctggac accacgacac
     3901 caggccctgc cctggaacag cttcagcagc tagagaaggc caaagctgca aagctagagc
     3961 aagagagaag agaggcagaa atgagggcaa aacgggaaga gggagagcga aagaggcagg
     4021 aagaactccg aagacaacag gaggaaattc ttcggcgaca gcaggaagaa gaaaggaaaa
     4081 ggcgagagga agaagaactt gtccgaagga aacaggaagg ggctctgcgt cgccagcggg
     4141 agcaagaaat tgcattaagg cgacagcgag aagaggaaga aagacagcag caagaagaag
     4201 ctcttagaag actggaagag aggagaagag aagaggaaga aaggcggaag caggaagaat
     4261 tgttacgcaa acaggaagag gaggctgcaa aatgggcccg ggaagaagga gaagcccagc
     4321 gtcgattaga ggagagccgg ctgcggatgg aagaggaggc agccagactc cggcatgagg
     4381 aagaagaacg gaagagaaag gagctggagg tccagcggca gaaggagtta atgcgccaga
     4441 ggcagcagca gcaagaggct ctccggaggt tgcagcagca gcagcagcaa caacagctgg
     4501 cgcagatgaa gcttccttct tcttcaacgt ggggccagca gtccaataca acagcatgtc
     4561 agtcccaggc cacgctgtcg ttggctgaaa tccaaaaact agaggaagaa cgagaacggc
     4621 agcttcgaga agagcaaagg cgccagcaga gggagttgat gaaagctctt cagcagcagc
     4681 agcaacagca acagcagaaa ctctcaggtt gggggaatgt cagcaaacct tcaggtacca
     4741 cgaaatctct tctggagatc cagcaggaag aggccaggca aatgcaaaag cagcagcagc
     4801 agcagcagca acaccagcaa ccaaacagag ctcgtaacaa tacgcattcc aacctgcaca
     4861 ccagcattgg gaattctgtt tggggctcta taaatactgg tcctcctaac cagtgggcat
     4921 ctgacctagt cagtagtatt tggagtaatg ctgacactaa aaactccaac atgggattct
     4981 gggatgatgc agtgaaagag gtgggaccta ggaattcaac aaataaaaat aaaaacaacg
     5041 ccagtctcag taaatctgta ggtgtgtcta accggcagaa taagaaagta gaagaagaag
     5101 aaaagttgct gaagctcttt cagggagtaa ataaagccca agatggattt acgcagtggt
     5161 gtgaacagat gcttcatgcc cttaatacgg caaataactt gggtgttccc acatttgttt
     5221 ctttcctgaa agaagtagaa tctccttatg aggtccatga ttatatcagg gcctatttag
     5281 gagatacttc tgaggccaag gagtttgcca agcagttcct tgagcgccgt gccaaacaga
     5341 aagccaacca gcagcgtcag cagcagcagc tgccacagca gcagcagcag cagccgccac
     5401 agcagccgcc acagcagcca caacagcagg actctgtgtg ggggatgaac cacagtacac
     5461 tccattcagt atttcagacc aatcaaagca acaaccaaca atccaatttt gaggctgtgc
     5521 agagtggcaa gaagaagaaa aagcagaaga tggtccgagc agatcccagt ttattaggat
     5581 tttcagtcaa tgcatcatcg gagcgactca acatgggtga aattgagacg ttggatgact
     5641 actagcttga cccagctttc ttgtacaaag tggtagatcc actagtccag tgtggtggaa
     5701 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc
     5761 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5821 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5881 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5941 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6001 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     6061 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6241 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6361 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6421 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     6481 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6541 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6601 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6661 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6721 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6781 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6841 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     6901 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     6961 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     7021 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     7081 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     7141 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     7201 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     7261 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     7321 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7381 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     7441 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7501 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7561 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     7621 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7681 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7741 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7801 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8581 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8641 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8701 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8761 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8821 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8881 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8941 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9001 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9061 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9121 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9181 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9241 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9301 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9361 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9421 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9481 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9541 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9601 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9661 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9721 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
