LOCUS       sequence-299760-        3571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt
            deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 E 27nt-del
FEATURES             Location/Qualifiers
     source          1..3571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(71..157)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    210..309
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(511..610)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(722..741)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(800..819)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1393..1926)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2115..2120,2121..2137,2138..2143))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2800..3388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3289..3308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3523..3550)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
       61 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
      121 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
      181 gcgttaacgc tagcatggat ctcgggcccc aaataatgat tttattttga ctgatagtga
      241 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      301 aagttggcat gtactctttc gtgaactccg tgctgctgtt cctggccttc gtggtgttcc
      361 tgctggtgac cctggctatc ctgaccgctc tgagactgtg cgcttactgc tgcaacatcg
      421 tgaacgtgtc cctggtgaag ccctctttct acgtgtacag ccgcgtgaag aacctgaaca
      481 gctccagggt gcctgacctg ctggtgtaat acccaacttt cttgtacaaa gttggcatta
      541 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      601 tcattatttg ccatccagct gcagctctgg cccgtgtctc aaaatctctg atgttacatt
      661 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
      721 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
      781 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
      841 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      901 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
      961 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1021 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1081 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1141 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1201 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     1261 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     1321 ttctcacttg ataaccttat ttttgacgag gggaaattag gccgggaagc cgatctcggc
     1381 ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
     1441 tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta
     1501 gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc
     1561 aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg
     1621 cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc
     1681 gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc
     1741 cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc
     1801 agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact
     1861 tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg
     1921 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     1981 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     2041 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     2101 gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca
     2161 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
     2221 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg
     2281 ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg
     2341 gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca
     2401 tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta
     2461 gtggttggct actaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     2521 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     2581 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     2641 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     2701 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tttcgttcca
     2761 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2821 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2881 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2941 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3001 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3061 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3121 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3181 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3241 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3301 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3361 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3421 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3481 taaccgtatt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     3541 atggccttct gcttagtttg atgcctggca g
//
