LOCUS       sequence-299772-        3714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP5 Cys146Ala
FEATURES             Location/Qualifiers
     source          1..3714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(124..151)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(243..329)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     378..400
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     392..409
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     393..409
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    425..524
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1449..1548)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1565..1584)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1566..1584)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1589..1605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1589..1605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2036..2827
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2920..3508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3409..3428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3662..3679
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
       61 gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac
      121 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
      181 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
      241 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      301 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
      361 gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg
      421 gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
      481 gcaatgcttt tttataatgc caactttgta caaaaaagtt ggcatgtccg gattccgcaa
      541 gatggccttc ccttctggca aggtggaggg ctgcatggtg caggtgacct gcggaaccac
      601 caccctgaac ggtctgtggc tggacgacgt ggtgtactgc cccaggcacg tgatctgcac
      661 ctctgaggac atgctgaacc ctaactacga agacctgctg atcagaaaga gcaaccacaa
      721 cttcctggtg caggccggta acgtgcagct gcgtgtgatc ggccactcta tgcagaactg
      781 cgtgctgaag ctgaaggtgg acaccgctaa ccccaagacc cctaagtaca agttcgtgag
      841 aatccagcct ggtcagacct tctccgtgct ggcctgctac aacggaagcc catccggcgt
      901 gtaccagtgc gctatgcgtc ccaacttcac catcaagggc agcttcctga acggctctgc
      961 cggaagcgtg ggtttcaaca tcgactacga ctgcgtgtcc ttctgctaca tgcaccacat
     1021 ggagctgcca actggagtgc acgctggaac tgacctggag ggtaacttct acggcccctt
     1081 cgtggacaga cagactgctc aggctgctgg caccgacacc accatcaccg tgaacgtgct
     1141 ggcttggctg tacgccgctg tgatcaacgg agaccgctgg ttcctgaaca ggttcaccac
     1201 caccctgaac gacttcaacc tggtggctat gaagtacaac tacgagcctc tgacccagga
     1261 ccacgtggat atcctgggtc cactgagcgc ccagactgga atcgctgtgc tggacatgtg
     1321 cgcctccctg aaggaactgc tgcagaacgg catgaacgga cgcaccatcc tgggatccgc
     1381 tctgctggag gacgaattca cccccttcga cgtggtgagg cagtgctctg gtgtgacctt
     1441 ccagtaatac ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     1501 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     1561 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     1621 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     1681 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     1741 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     1801 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     1861 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     1921 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     1981 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     2041 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     2101 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     2161 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     2221 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     2281 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     2341 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     2401 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     2461 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     2521 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     2581 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     2641 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     2701 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     2761 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     2821 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     2881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2941 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3061 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3241 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3541 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3601 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3661 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctct
//
